Reactivation of AKT signaling following treatment of cancer cells with PI3K inhibitors attenuates their antitumor effects.

Détails

ID Serval
serval:BIB_37BE773A7D11
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Reactivation of AKT signaling following treatment of cancer cells with PI3K inhibitors attenuates their antitumor effects.
Périodique
Biochemical and Biophysical Research Communications
Auteur(s)
Dufour M., Dormond-Meuwly A., Pythoud C., Demartines N., Dormond O.
ISSN
1090-2104 (Electronic)
ISSN-L
0006-291X
Statut éditorial
Publié
Date de publication
2013
Peer-reviewed
Oui
Volume
438
Numéro
1
Pages
32-37
Langue
anglais
Notes
Publication types: Journal Article
Résumé
Targeting the phosphatidylinositol-3-kinase (PI3K) is a promising approach in cancer therapy. In particular, PI3K blockade leads to the inhibition of AKT, a major downstream effector responsible for the oncogenic activity of PI3K. However, we report here that small molecule inhibitors of PI3K only transiently block AKT signaling. Indeed, treatment of cancer cells with PI3K inhibitors results in a rapid inhibition of AKT phosphorylation and signaling which is followed by the reactivation of AKT signaling after 48h as observed by Western blot. Reactivation of AKT signaling occurs despite effective inhibition of PI3K activity by PI3K inhibitors. In addition, wortmannin, a broad range PI3K inhibitor, did not block AKT reactivation suggesting that AKT signals independently of PI3K. In a therapeutical perspective, combining AKT and PI3K inhibitors exhibit stronger anti-proliferative and pro-apoptotic effects compared to AKT or PI3K inhibitors alone. Similarly, in a tumor xenograft mouse model, concomitant PI3K and AKT blockade results in stronger anti-cancer activity compared with either blockade alone. This study shows that PI3K inhibitors only transiently inhibit AKT which limits their antitumor activities. It also provides the proof of concept to combine PI3K inhibitors with AKT inhibitors in cancer therapy.
Pubmed
Web of science
Création de la notice
20/09/2013 18:23
Dernière modification de la notice
20/08/2019 14:26
Données d'usage