RNA polymerase II promoter-proximal pausing upregulates c-fos gene expression

Détails

ID Serval
serval:BIB_378D3ED4BCC7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
RNA polymerase II promoter-proximal pausing upregulates c-fos gene expression
Périodique
Gene
Auteur(s)
Fivaz  J., Bassi  M. C., Pinaud  S., Mirkovitch  J.
ISSN
0378-1119 (Print)
Statut éditorial
Publié
Date de publication
09/2000
Volume
255
Numéro
2
Pages
185-94
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Sep 19
Résumé
Transcription elongation regulates c-fos expression in mouse and human cells. In the inactive state of the gene, RNA polymerases are engaged only in the promoter-proximal region. Upon activation, RNA polymerases move efficiently along the complete gene. We have used Epstein-Barr virus (EBV) episomes as a gene transfer system to study the role of promoter-proximal pausing and transcript elongation in c-fos expression. We find that the sequence located immediately downstream of the transcriptional start site specifies pausing of RNA polymerases, dependent on both its orientation and position relative to the promoter. This sequence is, however, not necessary to maintain repression in the absence of a stimulus. As promoter-proximal pausing is therefore not a repression mechanism for the c-fos gene, the promoter and enhancer sequences are the main determinants of RNA polymerase elongation competence. Surprisingly, we find that promoter-proximal pausing further increases transcriptional levels from a variety of promoters. These observations lead us to hypothesize that promoter-proximal pausing of RNA polymerase II augments c-fos expression by allowing more efficient phosphorylation of the C-terminal domain of the large subunit.
Mots-clé
Animals Cell Line DNA/genetics/metabolism Gene Expression Regulation/drug effects Herpesvirus 4, Human/genetics Humans Jurkat Cells Luciferases/genetics/metabolism Mice Plasmids/genetics Promoter Regions (Genetics)/*genetics Proto-Oncogene Proteins c-fos/*genetics RNA/drug effects/genetics/metabolism RNA Polymerase II/*metabolism Recombinant Fusion Proteins/drug effects/genetics/metabolism Tetradecanoylphorbol Acetate/pharmacology Transcription, Genetic Tumor Cells, Cultured Up-Regulation
Pubmed
Web of science
Création de la notice
25/01/2008 9:41
Dernière modification de la notice
20/08/2019 14:26
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