Cysteine-rich regions of protein kinase Cdelta are functionally non-equivalent. Differences between cysteine-rich regions of non-calcium-dependent protein kinase Cdelta and calcium-dependent protein kinase Cgamma.

Détails

ID Serval
serval:BIB_372
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Cysteine-rich regions of protein kinase Cdelta are functionally non-equivalent. Differences between cysteine-rich regions of non-calcium-dependent protein kinase Cdelta and calcium-dependent protein kinase Cgamma.
Périodique
Febs Letters
Auteur⸱e⸱s
Hunn M., Quest A.F.
ISSN
0014-5793 (Print)
ISSN-L
0014-5793
Statut éditorial
Publié
Date de publication
1997
Volume
400
Numéro
2
Pages
226-232
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Regulatory domain elements of the non-calcium-dependent protein kinase Cdelta (nPKCdelta), including either or both of the cysteine-rich regions Cys1(delta) and Cys2(delta), were expressed as fusion proteins with glutathione-S-transferase and characterized using liposomal or mixed micellar phorbol ester binding assays. Fusion proteins containing Cys2(delta) bound phorbol-12,13-dibutyrate (PDBu) efficiently in the assay employing phosphatidylserine (PS) vesicles, while no significant binding was seen for proteins containing only Cys1(delta). Likewise, in mixed micellar assays, fusion proteins with Cys2(delta) bound PDBu with high affinity (Kd: 14-37 nM) and to significant stoichiometric levels (0.23-0.66 mol/mol), but no binding could be detected for proteins with Cys1(delta) only. The PS dependence of PDBu binding to Cys2(delta) was highly cooperative with Hill numbers lying in the range of 2.5-5.2. These results demonstrate the presence of striking functional differences between the cysteine-rich regions of nPKCdelta and the calcium-dependent isoform, cPKCgamma, where both cysteine-rich regions represent functional PDBu binding elements.
Mots-clé
Binding Sites, Calcium/pharmacology, Cysteine, Dose-Response Relationship, Drug, Glutathione Transferase/genetics, Isoenzymes/genetics, Isoenzymes/isolation & purification, Micelles, Phorbol 12,13-Dibutyrate/metabolism, Phosphatidylserines/metabolism, Protein Kinase C/genetics, Protein Kinase C/isolation & purification, Protein Kinase C-delta, Recombinant Fusion Proteins/genetics, Recombinant Fusion Proteins/isolation & purification, Structure-Activity Relationship
Pubmed
Web of science
Création de la notice
19/11/2007 13:35
Dernière modification de la notice
20/08/2019 14:25
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