IgM autoantibodies to 180- and 230- to 240-kd human epidermal proteins in pregnancy

Détails

ID Serval
serval:BIB_3654225DB184
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
IgM autoantibodies to 180- and 230- to 240-kd human epidermal proteins in pregnancy
Périodique
Archives of Dermatology
Auteur(s)
Borradori  L., Didierjean  L., Bernard  P., Tamm  K., Gaucherand  M., Zurn  A., Saurat  J. H.
ISSN
0003-987X (Print)
Statut éditorial
Publié
Date de publication
01/1995
Volume
131
Numéro
1
Pages
43-7
Notes
Journal Article --- Old month value: Jan
Résumé
BACKGROUND AND DESIGN: A previous study has suggested that there is a novel entity among the polymorphous eruptions of pregnancy (PEP) associated with circulating anti-basement membrane zone IgM autoantibodies. To determine if the presence of anti-basement membrane zone IgM autoantibodies is a feature of PEP, serum samples from 52 patients with a PEP, 69 healthy pregnant women, and 42 nonpregnant women were prospectively evaluated by indirect immunofluorescence using salt-split human skin as substrate. Serum samples were also tested by immunoblotting using keratinocyte extracts and anti-human IgM antibodies. The reactivity of some serum samples was examined using two recombinant bullous pemphigoid antigen proteins. RESULTS: The percentage of women with a PEP, healthy pregnant women, and nonpregnant women who had anti-basement membrane zone IgM antibodies by indirect immunofluorescence was similar: 12%, 10%, and 14% of cases, respectively. By immunoblotting, 14% of the serum samples from the patients with a PEP, 12% of the serum samples from the healthy pregnant women, but only 2% of the serum samples from the nonpregnant women contained IgM antibodies that reacted with epidermal proteins of 180 and/or 230 to 240 kd. The recombinant bullous pemphigoid antigen proteins were not recognized by any of the serum samples that showed a reactivity by immunoblotting using keratinocyte extracts. CONCLUSION: There is no evidence for the existence of a novel entity of pregnancy defined by circulating anti-basement membrane zone IgM autoantibodies. Immunoblotting detects IgM autoantibodies that react with epidermal proteins of 180 and/or 230 to 240 kd. These autoantibodies appear to be more frequent in pregnant than in nonpregnant women. Although the nature of the target antigen(s) remains to be established, pregnancy may be associated with low levels of IgM autoreactivity against epidermal proteins.
Mots-clé
Adult Autoantibodies/*metabolism Basement Membrane/*metabolism Case-Control Studies Female Fluorescent Antibody Technique Humans Immunoglobulin G/*metabolism Immunoglobulin M/*metabolism Keratinocytes/*metabolism Middle Aged Molecular Weight Pregnancy Pregnancy Complications/*metabolism Pregnancy Proteins/*immunology Prospective Studies Protein Binding Skin Diseases/*metabolism
Pubmed
Web of science
Création de la notice
28/01/2008 8:44
Dernière modification de la notice
20/08/2019 13:24
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