Epigenetic features of human mesenchymal stem cells determine their permissiveness for induction of relevant transcriptional changes by SYT-SSX1.

Détails

Ressource 1Télécharger: BIB_35F298DAEEF2.P001.pdf (1261.01 [Ko])
Etat: Public
Version: Final published version
ID Serval
serval:BIB_35F298DAEEF2
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Epigenetic features of human mesenchymal stem cells determine their permissiveness for induction of relevant transcriptional changes by SYT-SSX1.
Périodique
Plos One
Auteur⸱e⸱s
Cironi L., Provero P., Riggi N., Janiszewska M., Suva D., Suva M.L., Kindler V., Stamenkovic I.
ISSN
1932-6203[electronic], 1932-6203[linking]
Statut éditorial
Publié
Date de publication
2009
Peer-reviewed
Oui
Volume
4
Numéro
11
Pages
e7904
Langue
anglais
Notes
Publication types: Journal Article
Résumé
BACKGROUND: A characteristic SYT-SSX fusion gene resulting from the chromosomal translocation t(X;18)(p11;q11) is detectable in almost all synovial sarcomas, a malignant soft tissue tumor widely believed to originate from as yet unidentified pluripotent stem cells. The resulting fusion protein has no DNA binding motifs but possesses protein-protein interaction domains that are believed to mediate association with chromatin remodeling complexes. Despite recent advances in the identification of molecules that interact with SYT-SSX and with the corresponding wild type SYT and SSX proteins, the mechanisms whereby the SYT-SSX might contribute to neoplastic transformation remain unclear. Epigenetic deregulation has been suggested to be one possible mechanism. METHODOLOGY/PRINCIPAL FINDINGS: We addressed the effect of SYT/SSX expression on the transcriptome of four independent isolates of primary human bone marrow mesenchymal stem cells (hMSC). We observed transcriptional changes similar to the gene expression signature of synovial sarcoma, principally involving genes whose regulation is linked to epigenetic factors, including imprinted genes, genes with transcription start sites within a CpG island and chromatin related genes. Single population analysis revealed hMSC isolate-specific transcriptional changes involving genes that are important for biological functions of stem cells as well as genes that are considered to be molecular markers of synovial sarcoma including IGF2, EPHRINS, and BCL2. Methylation status analysis of sequences at the H19/IGF2 imprinted locus indicated that distinct epigenetic features characterize hMSC populations and condition the transcriptional effects of SYT-SSX expression. CONCLUSIONS/SIGNIFICANCE: Our observations suggest that epigenetic features may define the cellular microenvironment in which SYT-SSX displays its functional effects.
Mots-clé
Adolescent, Alleles, Child, Chromatin/metabolism, CpG Islands, DNA/genetics, Epigenesis, Genetic, Gene Expression Profiling, Gene Expression Regulation, Humans, Mesenchymal Stem Cells/cytology, Oncogene Proteins, Fusion/genetics, Oncogene Proteins, Fusion/physiology, Sarcoma, Synovial/metabolism, Transcription, Genetic, Translocation, Genetic
Pubmed
Web of science
Open Access
Oui
Création de la notice
19/01/2010 17:48
Dernière modification de la notice
20/08/2019 14:23
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