Versatile controlling system for cryopreparation techniques in electron microscopy.
Détails
ID Serval
serval:BIB_35A15AAC1E7E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Versatile controlling system for cryopreparation techniques in electron microscopy.
Périodique
Journal of Electron Microscopy Technique
ISSN
1059-910X
ISSN-L
0741-0581
Statut éditorial
Publié
Date de publication
1991
Volume
17
Numéro
4
Pages
450-455
Langue
anglais
Résumé
A new setup for freeze-substitution and a versatile controlling system has been developed. Our goal was to build a simple system allowing precise control of the physical parameters of freeze-substitution experiments to learn more about their influences on the cellular ultrastructure and immunoreactivity of macromolecules. An improved apparatus for freeze-substitution, based on liquid nitrogen cooling, and a universal software for controlling the complex preparation protocols from cryofixation to final polymerization are described. This controlling system has the following advantages: it allows precise control and registration of temperature profiles, reconstruction of each individual step of previous experiments, and optimization of working conditions. The setup of the freeze-substitution apparatus is designed to run many different substitution media in parallel; freeze-substitution (cryostat), embedding (working platform), and polymerization are carried out at separate places; therefore, more experiments can be done simultaneously. The ergonomic working platform allows exchange of media at controlled temperature and easy handling; survey of the temperature in individual tubes is possible, and the system is protected from water condensation and uncontrolled warming by the deep freezer.
Mots-clé
Computers, Cryopreservation, Microscopy, Electron, Software
Pubmed
Web of science
Création de la notice
18/10/2012 13:12
Dernière modification de la notice
20/08/2019 13:23