Versatile controlling system for cryopreparation techniques in electron microscopy.

Détails

ID Serval
serval:BIB_35A15AAC1E7E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Versatile controlling system for cryopreparation techniques in electron microscopy.
Périodique
Journal of Electron Microscopy Technique
Auteur⸱e⸱s
Humbel B.M., Weber K., Merkl R.
ISSN
1059-910X
ISSN-L
0741-0581
Statut éditorial
Publié
Date de publication
1991
Volume
17
Numéro
4
Pages
450-455
Langue
anglais
Résumé
A new setup for freeze-substitution and a versatile controlling system has been developed. Our goal was to build a simple system allowing precise control of the physical parameters of freeze-substitution experiments to learn more about their influences on the cellular ultrastructure and immunoreactivity of macromolecules. An improved apparatus for freeze-substitution, based on liquid nitrogen cooling, and a universal software for controlling the complex preparation protocols from cryofixation to final polymerization are described. This controlling system has the following advantages: it allows precise control and registration of temperature profiles, reconstruction of each individual step of previous experiments, and optimization of working conditions. The setup of the freeze-substitution apparatus is designed to run many different substitution media in parallel; freeze-substitution (cryostat), embedding (working platform), and polymerization are carried out at separate places; therefore, more experiments can be done simultaneously. The ergonomic working platform allows exchange of media at controlled temperature and easy handling; survey of the temperature in individual tubes is possible, and the system is protected from water condensation and uncontrolled warming by the deep freezer.
Mots-clé
Computers, Cryopreservation, Microscopy, Electron, Software
Pubmed
Web of science
Création de la notice
18/10/2012 13:12
Dernière modification de la notice
20/08/2019 13:23
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