Kinetics of synaptotagmin responses to Ca2+ and assembly with the core SNARE complex onto membranes.

Détails

ID Serval
serval:BIB_3557D8163CE1
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Kinetics of synaptotagmin responses to Ca2+ and assembly with the core SNARE complex onto membranes.
Périodique
Neuron
Auteur(s)
Davis A.F., Bai J., Fasshauer D., Wolowick M.J., Lewis J.L., Chapman E.R.
ISSN
0896-6273 (Print)
ISSN-L
0896-6273
Statut éditorial
Publié
Date de publication
1999
Peer-reviewed
Oui
Volume
24
Numéro
2
Pages
363-376
Langue
anglais
Résumé
The synaptic vesicle protein synaptotagmin I binds Ca2+ and is required for efficient neurotransmitter release. Here, we measure the response time of the C2 domains of synaptotagmin to determine whether synaptotagmin is fast enough to function as a Ca2+ sensor for rapid exocytosis. We report that synaptotagmin is "tuned" to sense Ca2+ concentrations that trigger neuronal exocytosis. The speed of response is unique to synaptotagmin I and readily satisfies the kinetic constraints of synaptic vesicle membrane fusion. We further demonstrate that Ca2+ triggers penetration of synaptotagmin into membranes and simultaneously drives assembly of synaptotagmin onto the base of the ternary SNARE (soluble N-ethylmaleimide-sensitive fusion protein [NSF] attachment receptor) complex, near the transmembrane anchor of syntaxin. These data support a molecular model in which synaptotagmin triggers exocytosis through its interactions with membranes and the SNARE complex.
Mots-clé
Animals, Calcium/physiology, Calcium-Binding Proteins, Kinetics, Membrane Fusion/physiology, Membrane Glycoproteins/chemistry, Membrane Glycoproteins/genetics, Membrane Proteins/metabolism, Membranes/metabolism, Nerve Tissue Proteins/chemistry, Nerve Tissue Proteins/genetics, Rats, Repetitive Sequences, Nucleic Acid, SNARE Proteins, Sequence Homology, Synaptic Vesicles/metabolism, Synaptotagmin I, Synaptotagmins, Vesicular Transport Proteins
Pubmed
Web of science
Open Access
Oui
Création de la notice
15/09/2011 10:30
Dernière modification de la notice
20/08/2019 14:22
Données d'usage