Multiple adenosine 3',5'-cyclic [corrected] monophosphate response element DNA-binding proteins generated by gene diversification and alternative exon splicing.

Détails

ID Serval
serval:BIB_3521DE0CF761
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Multiple adenosine 3',5'-cyclic [corrected] monophosphate response element DNA-binding proteins generated by gene diversification and alternative exon splicing.
Périodique
Molecular Endocrinology
Auteur(s)
Hoeffler J.P., Meyer T.E., Waeber G., Habener J.F.
ISSN
0888-8809
Statut éditorial
Publié
Date de publication
1990
Peer-reviewed
Oui
Volume
4
Numéro
6
Pages
920-930
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article
Résumé
The protein sequence deduced from the open reading frame of a human placental cDNA encoding a cAMP-responsive enhancer (CRE)-binding protein (CREB-327) has structural features characteristic of several other transcriptional transactivator proteins including jun, fos, C/EBP, myc, and CRE-BP1. Results of Southwestern analysis of nuclear extracts from several different cell lines show that there are multiple CRE-binding proteins, which vary in size in cell lines derived from different tissues and animal species. To examine the molecular diversity of CREB-327 and related proteins at the nucleic acid level, we used labeled cDNAs from human placenta that encode two different CRE-binding proteins (CREB-327 and CRE-BP1) to probe Northern and Southern blots. Both probes hybridized to multiple fragments on Southern blots of genomic DNA from various species. Alternatively, when a human placental c-jun probe was hybridized to the same blot, a single fragment was detected in most cases, consistent with the intronless nature of the human c-jun gene. The CREB-327 probe hybridized to multiple mRNAs, derived from human placenta, ranging in size from 2-9 kilobases. In contrast, the CRE-BP1 probe identified a single 4-kilobase mRNA. Sequence analyses of several overlapping human genomic cosmid clones containing CREB-327 sequences in conjunction with polymerase chain reaction indicates that the CREB-327/341 cDNAs are composed of at least eight or nine exons, and analyses of human placental cDNAs provide direct evidence for at least one alternatively spliced exon. Analyses of mouse/hamster-human hybridoma DNAs by Southern blotting and polymerase chain reaction localizes the CREB-327/341 gene to human chromosome 2. The results indicate that there is a dichotomy of CREB-like proteins, those that are related by overall structure and DNA-binding specificity as well as those that are related by close similarities of primary sequences.
Mots-clé
Amino Acid Sequence, Animals, Base Sequence, Chromosome Mapping, Chromosomes, Human, Pair 2, Cyclic AMP Response Element-Binding Protein, DNA/analysis, DNA/genetics, DNA-Binding Proteins/genetics, DNA-Binding Proteins/metabolism, Exons, Female, Genetic Variation/genetics, Humans, Molecular Sequence Data, Peptide Fragments/genetics, Peptide Fragments/metabolism, Placenta/metabolism, Pregnancy, RNA Splicing/genetics, RNA, Messenger/genetics, RNA, Messenger/metabolism, Rats, Transcription, Genetic/genetics
Pubmed
Web of science
Création de la notice
25/01/2008 14:10
Dernière modification de la notice
20/08/2019 13:22
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