Junctional adhesion molecule-2 (JAM-2) promotes lymphocyte transendothelial migration.

Détails

ID Serval
serval:BIB_3503717C64CA
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Junctional adhesion molecule-2 (JAM-2) promotes lymphocyte transendothelial migration.
Périodique
Blood
Auteur(s)
Johnson-Léger C.A., Aurrand-Lions M., Beltraminelli N., Fasel N., Imhof B.A.
ISSN
0006-4971 (Print)
ISSN-L
0006-4971
Statut éditorial
Publié
Date de publication
2002
Volume
100
Numéro
7
Pages
2479-2486
Langue
anglais
Résumé
The molecular mechanisms underlying lymphocyte extravasation remain poorly characterized. We have recently identified junctional adhesion molecule-2 (JAM-2), and have shown that antibodies to JAM-2 stain high endothelial venules (HEVs) within lymph nodes and Peyer patches of adult mice. Here we show that mouse lymphocytes migrate in greater numbers across monolayers of endothelioma cells transfected with JAM-2. The significance of these findings to an understanding of both normal and pathologic lymphocyte extravasation prompted us to clone the human homologue of JAM-2. We herein demonstrate that an anti-JAM-2 antibody, or a soluble JAM-2 molecule, blocks the transmigration of primary human peripheral blood leukocytes across human umbilical vein endothelial cells expressing endogenous JAM-2. Furthermore, we show that JAM-2 is expressed on HEVs in human tonsil and on a subset of human leukocytes, suggesting that JAM-2 plays a central role in the regulation of transendothelial migration.
Mots-clé
Amino Acid Sequence, Animals, Antibodies, Monoclonal/pharmacology, Base Sequence, Cell Adhesion Molecules/drug effects, Cell Adhesion Molecules/genetics, Cell Line, Cell Movement/drug effects, Cell Movement/physiology, Endothelium, Vascular/physiology, Gene Expression Regulation/physiology, Humans, Immunoglobulins/drug effects, Immunoglobulins/genetics, Lymphocytes/physiology, Membrane Proteins/drug effects, Membrane Proteins/genetics, Mice, Molecular Sequence Data, Sequence Alignment, Sequence Homology, Amino Acid
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 16:02
Dernière modification de la notice
20/08/2019 14:22
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