Myelin gene expression during demyelination and remyelination in aggregating brain cell cultures.

Détails

ID Serval
serval:BIB_34B3E099E4B7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Myelin gene expression during demyelination and remyelination in aggregating brain cell cultures.
Périodique
Journal of Neuroimmunology
Auteur(s)
Matthieu J.M., Comte V., Tosic M., Honegger P.
ISSN
0165-5728 (Print)
ISSN-L
0165-5728
Statut éditorial
Publié
Date de publication
1992
Volume
40
Numéro
2-3
Pages
231-234
Langue
anglais
Résumé
Remyelination can be studied in aggregating rat brain cell cultures after limited demyelination. Demyelination was induced using a monoclonal antibody against myelin/oligodendrocyte glycoprotein (MOG mAb), in the presence of complement. De- and remyelination were assessed by measuring myelin basic protein (MBP). Two days after removing the MOG mAb, MBP levels reached 50% of controls and after 7 days 93%. During this period, cell proliferation determined by [14C]thymidine incorporation was similar in remyelinating and control cultures. Hormones and growth factors were tested for possible stimulatory effect on remyelinating cultures. Bovine growth hormone (bGH), triiodothyronine (T3), basic fibroblast growth factor (bFGF) and platelet-derived growth factor (PDGF) did not improve remyelination. Only epidermal growth factor (EGF) increased the level of remyelination. PDGF increased the rate of cell proliferation in both control and remyelinating cultures. A significant proportion of oligodendrocytes entered the cell division cycle and were not available for remyelination. The results obtained with PDGF and FGF (inhibition) support the idea that a pool of progenitor cells was still present and able to proliferate and differentiate into myelinating oligodendrocytes. The levels of myelin protein mRNAs were investigated during de- and remyelination. During demyelination, myelin protein mRNA levels decreased to approximately 50% of control cultures and returned to normal during remyelination. These preliminary results indicate that normal levels of gene transcription are sufficient to meet the increased need for newly synthesized myelin proteins during remyelination.
Mots-clé
2',3'-Cyclic-Nucleotide Phosphodiesterases/genetics, 2',3'-Cyclic-Nucleotide Phosphodiesterases/metabolism, Animals, Brain/cytology, Brain/metabolism, Cattle, Cell Aggregation, Cells, Cultured, Demyelinating Diseases/genetics, Growth Hormone/pharmacology, Growth Substances/pharmacology, Myelin Basic Protein/genetics, Myelin Basic Protein/metabolism, Myelin Sheath/drug effects, Myelin Sheath/physiology, RNA, Messenger/metabolism, Rats, Triiodothyronine/pharmacology
Pubmed
Web of science
Création de la notice
24/01/2008 14:11
Dernière modification de la notice
20/08/2019 14:21
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