Inducible nitric oxide synthase-dependent stimulation of PKGI and phosphorylation of VASP in human embryonic kidney cells.

Détails

ID Serval
serval:BIB_34965
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Inducible nitric oxide synthase-dependent stimulation of PKGI and phosphorylation of VASP in human embryonic kidney cells.
Périodique
Biochemical Pharmacology
Auteur(s)
André M., Latado H., Felley-Bosco E.
ISSN
0006-2952
Statut éditorial
Publié
Date de publication
2005
Volume
69
Numéro
4
Pages
595-602
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Résumé
Inducible nitric oxide synthase (iNOS) production of nitric oxide (NO) has been mostly associated with so-called nitrosative stress or interaction with superoxide anion. However, recent investigations have indicated that, as for the other isoenzymes producing NO, guanylyl cyclase (GC) is a very sensitive target of iNOS activity. To further investigate this less explored signaling, the NO-cyclic guanosine 3'-5'-monophosphate (NO-cGMP)-induced vasodilator-stimulated phosphoprotein (VASP) phosphorylation on serine 239 was investigated in human embryonic kidney 293 cells (HEK cells). First, the expression and activity of alpha2 and beta1 NO-sensitive GC subunits was determined by Western blot analysis, reverse transcription-polymerase chain reaction and NO donors administration. Then, the expression of a functional cGMP-dependent protein kinase I (PKGI) was verified by addition of 8-Br-cGMP followed by determination of phosphorylation of VASP on serine 239. Finally, iNOS activation of this signaling pathway was characterized after transfection of HEK cells with human iNOS cDNA. Altogether our data show that iNOS-derived NO activates endogenous NO-sensitive GC and leads to VASP phosphorylation in HEK cells.
Mots-clé
Amino Acid Sequence, Cell Adhesion Molecules, Cells, Cultured, Cyclic GMP, Cyclic GMP-Dependent Protein Kinases, Humans, Microfilament Proteins, Molecular Sequence Data, Nitric Oxide, Nitric Oxide Donors, Nitric Oxide Synthase, Nitric Oxide Synthase Type II, Phosphoproteins, Phosphorylation, Signal Transduction
Pubmed
Web of science
Création de la notice
19/11/2007 13:33
Dernière modification de la notice
20/08/2019 14:21
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