Induction of a cytotoxic T-cell response to HIV-1 proteins with short synthetic peptides and human compatible adjuvants
Détails
ID Serval
serval:BIB_347FD9650034
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Induction of a cytotoxic T-cell response to HIV-1 proteins with short synthetic peptides and human compatible adjuvants
Périodique
Vaccine
ISSN
0264-410X (Print)
Statut éditorial
Publié
Date de publication
07/2001
Volume
19
Numéro
30
Pages
4121-9
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jul 20
Research Support, Non-U.S. Gov't --- Old month value: Jul 20
Résumé
The goal of this study was the induction of a strong CTL response against multiple CTL epitopes present in HIV proteins using short synthetic peptides. Four HLA-A2.1 restricted peptides (RT 476-484, p17 77-85, gp41 814-823, RT 956-964) that showed stable binding to the HLA-A2.1 molecule in an in vitro binding assay were able to elicit a strong specific immune response in HLA-A2.1 transgenic mice when injected with IFA or Montanide. The use of biodegradable microspheres (MS) as adjuvant was also successfully tested for all peptides. When the peptides were injected as a mixture the response was weaker as compared to individual injections of the peptides indicating the occurrence of immunodominance (ID). We are currently investigating whether ID can be overcome by a combined injection of peptide loaded MS with different release patterns. Taken together, it seems feasible to induce a specific CTL response in HLA-A2.1 transgenic mice against several HIV proteins using short synthetic peptides and human compatible adjuvants.
Mots-clé
AIDS Vaccines/*immunology
Adjuvants, Immunologic/*administration & dosage
Amino Acid Sequence
Animals
Cell Line
Female
HIV-1/*immunology
HLA-A2 Antigen/*physiology
Humans
Immunization
Male
Mice
Mice, Transgenic
Microspheres
Molecular Sequence Data
Peptide Fragments/*immunology
T-Lymphocytes, Cytotoxic/*immunology
Pubmed
Web of science
Création de la notice
24/01/2008 15:55
Dernière modification de la notice
20/08/2019 14:21