Sequence of the gene coding for ribosomal protein S8 of Xenopus laevis.

Détails

ID Serval
serval:BIB_3459A02B93BF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Sequence of the gene coding for ribosomal protein S8 of Xenopus laevis.
Périodique
Gene
Auteur(s)
Mariottini P., Bagni C., Francesconi A., Cecconi F., Serra M.J., Chen Q.M., Loreni F., Annesi F., Amaldi F.
ISSN
0378-1119 (Print)
ISSN-L
0378-1119
Statut éditorial
Publié
Date de publication
15/10/1993
Volume
132
Numéro
2
Pages
255-260
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
We present here the cloning and the entire sequence of one of the two gene copies coding for ribosomal protein (r-protein) S8 in Xenopus laevis (corresponding to r-protein S7 in rat) and its flanking regions. The S8a gene contains seven exons and six introns for a total length of about 12,700 bp coding for a mRNA of 663 nucleotides (nt) plus a poly(A) tail. Mapping of the 5' end of the gene has shown that the transcription start point is located in a pyrimidine-rich tract, as has been observed for all r-protein-encoding genes of X. laevis and other vertebrates so far characterized. A computer analysis of the S8a sequence has revealed the presence of a 220-nt sequence repeated, with some variations, once in each of the six introns. RNA analysis by hybridization with oligo probes specific for the two gene copies coding for r-protein S8 has demonstrated that the two of them are expressed at similar levels both in oocytes and in embryos.

Mots-clé
Animals, Base Sequence, Blotting, Southern, Cloning, Molecular, DNA, Exons, Introns, Molecular Sequence Data, Restriction Mapping, Ribosomal Proteins/genetics, Xenopus laevis/genetics
Pubmed
Création de la notice
06/03/2017 18:23
Dernière modification de la notice
20/08/2019 14:21
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