Two genes contribute to different extents to the heme oxygenase enzyme activity measured in cultured human skin fibroblasts and keratinocytes: implications for protection against oxidant stress

Détails

ID Serval
serval:BIB_344E5681B1D6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Two genes contribute to different extents to the heme oxygenase enzyme activity measured in cultured human skin fibroblasts and keratinocytes: implications for protection against oxidant stress
Périodique
Photochemistry and Photobiology
Auteur(s)
Applegate  L. A., Noel  A., Vile  G., Frenk  E., Tyrrell  R. M.
ISSN
0031-8655 (Print)
Statut éditorial
Publié
Date de publication
1995
Volume
61
Numéro
3
Pages
285-291
Notes
DA - 19950512
LA - eng
PT - Journal Article
PT - Research Support, Non-U.S. Gov't
RN - 0 (Oxidants)
RN - 0 (RNA, Messenger)
RN - 7722-84-1 (Hydrogen Peroxide)
RN - EC 1.14.99.3 (Heme Oxygenase (Decyclizing))
SB - IM
Résumé
Activation of expression of the heme oxygenase (HO) gene appears to be involved in a cellular defense system in mammalian cells. We now demonstrate that while HO-1 mRNA levels are strongly inducible in dermal fibroblasts they are barely inducible in human epidermal keratinocytes following oxidative stress (UVA radiation and hydrogen peroxide). Paralleling this result was the observation that HO-2 mRNA levels were low in dermal fibroblasts but were high in epidermal keratinocytes. In neither case was the HO-2 gene inducible. The expression of the two HO genes led to enzymatic activity in both types of skin cells with an approximately 2.5-fold higher level of enzymatic activity present in keratinocytes compared with fibroblasts derived from the same biopsy. In addition, ferritin levels, which have been found to be augmented via the HO-dependent release of iron from endogenous heme sources, were two- to three-fold higher in keratinocytes compared with matching fibroblasts. This higher ferritin pool would result in an enhancement of cellular iron sequestering capacity that may confer increased resistance to oxidative stress. Indeed, keratinocytes showed less UVA radiation-dependent cell membrane damage than fibroblasts. These results are consistent with the hypothesis that HO expression in human epidermis and dermis is related to cellular defense mechanisms that operate in human skin
Mots-clé
Biopsy/Cell Membrane/Cells,Cultured/cytology/drug effects/enzymology/Fibroblasts/genetics/Heme Oxygenase (Decyclizing)/Humans/Hydrogen Peroxide/Keratinocytes/metabolism/Oxidants/Oxidative Stress/pharmacology/radiation effects/RNA,Messenger/Skin/Switzerland/Ultraviolet Rays
Pubmed
Web of science
Création de la notice
18/02/2008 18:33
Dernière modification de la notice
20/08/2019 14:20
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