Molecular control of tissue-specific expression at the mouse TNF locus

Détails

ID Serval
serval:BIB_3382B700997A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Molecular control of tissue-specific expression at the mouse TNF locus
Périodique
European Journal of Immunology
Auteur(s)
Jongeneel  C. V., Shakhov  A. N., Nedospasov  S. A., Cerottini  J. C.
ISSN
0014-2980 (Print)
Statut éditorial
Publié
Date de publication
03/1989
Volume
19
Numéro
3
Pages
549-52
Notes
Journal Article --- Old month value: Mar
Résumé
We have examined the patterns of mRNA accumulation and transcription of the tandemly linked genes for tumor necrosis factor (TNF)-beta and TNF-alpha. In spite of their tandem arrangement and close linkage, the two TNF genes utilize separate promoters. Our results show that, while levels of mRNA correlate with patterns of TNF-alpha and TNF-beta secretion by lymphocytes and macrophages, the transcriptional levels of the corresponding genes do not. The TNF-alpha gene is transcribed much more heavily than the TNF-beta gene in T lymphocytes, even though the TNF-beta mRNA is more abundant. Resting T lymphocytes and macrophages, which do not accumulate any TNF mRNA, nevertheless transcribe the TNF-alpha gene actively. On the other hand, the TNF-beta gene is transcriptionally silent in macrophages. Our results are consistent with a model where tissue specificity is controlled transcriptionally, whereas post-transcriptional events differentially control mRNA abundance.
Mots-clé
Base Sequence Chromosome Mapping Humans Macrophages/metabolism Promoter Regions (Genetics) RNA, Messenger/analysis T-Lymphocytes, Cytotoxic/metabolism Transcription, Genetic Tumor Necrosis Factor-alpha/*genetics
Pubmed
Web of science
Création de la notice
24/01/2008 15:39
Dernière modification de la notice
20/08/2019 13:19
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