Analytical validation of a lymphocytic choriomeningitis virus real-time RT-PCR assay.

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Ressource 1Télécharger: BIB_336A7319E9E2.P001.pdf (778.17 [Ko])
Etat: Public
Version: Final published version
ID Serval
serval:BIB_336A7319E9E2
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Analytical validation of a lymphocytic choriomeningitis virus real-time RT-PCR assay.
Périodique
Journal of Virological Methods
Auteur(s)
Cordey S., Sahli R., Moraz M.L., Estrade C., Morandi L., Cherpillod P., Charrel R.N., Kunz S., Kaiser L.
ISSN
1879-0984 (Electronic)
ISSN-L
0166-0934
Statut éditorial
Publié
Date de publication
2011
Volume
177
Numéro
1
Pages
118-22
Langue
anglais
Résumé
Lymphocytic choriomeningitis virus (LCMV) is a rare cause of central nervous system disease in humans. Screening by real-time RT-PCR assay is of interest in the case of aseptic meningitis of unknown etiology. A specific LCMV real-time RT-PCR assay, based on the detection of genomic sequences of the viral nucleoprotein (NP), was developed to assess the presence of LCMV in cerebrospinal fluids (CSF) sent for viral screening to a Swiss university hospital laboratory. A 10-fold dilution series assay using a plasmid containing the cDNA of the viral NP of the LCMV isolate Armstrong (Arm) 53b demonstrated the high sensitivity of the assay with a lowest detection limit of ≤50 copies per reaction. High sensitivity was confirmed by dilution series assays in a pool of human CSF using four different LCMV isolates (Arm53b, WE54, Traub and E350) with observed detection limits of ≤10PFU/ml (Arm53b and WE54) and 1PFU/ml (Traub and E350). Analysis of 130 CSF showed no cases of acute infection. The absence of positive cases was confirmed by a published PCR assay detecting all Old World arenaviruses. This study validates a specific and sensitive real-time RT-PCR assay for the diagnosis of LCMV infections. Results showed that LCMV infections are extremely rare in hospitalized patients western in Switzerland.
Pubmed
Web of science
Open Access
Oui
Création de la notice
13/10/2011 11:27
Dernière modification de la notice
20/08/2019 14:19
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