Effects of mitotane on gene expression in the adrenocortical cell line NCI-H295R: a microarray study.

Détails

ID Serval
serval:BIB_33681841C3BB
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Effects of mitotane on gene expression in the adrenocortical cell line NCI-H295R: a microarray study.
Périodique
Pharmacogenomics
Auteur(s)
Zsippai A., Szabó D.R., Tömböl Z., Szabó P.M., Eder K., Pállinger E., Gaillard R.C., Patócs A., Tóth S., Falus A., Rácz K., Igaz P.
ISSN
1744-8042 (Electronic)
ISSN-L
1462-2416
Statut éditorial
Publié
Date de publication
2012
Volume
13
Numéro
12
Pages
1351-1361
Langue
anglais
Notes
Publication types: Journal ArticlePublication Status: ppublish
Résumé
Aim: The adrenolytic agent mitotane is widely used in the treatment of adrenocortical cancer; however, its mechanism of action is poorly elucidated. We have studied mitotane-induced mRNA expression changes in the NCI-H295R adrenocortical cancer cell line. Materials & methods: Cell viability and hormone assays were used to select the optimal mitotane concentration effectively inhibiting hormone secretion without affecting cell viability. RNA isolated from cultures treated for 48 and 72 h was subjected to Agilent 4×44K microarray platforms. Microarray results were validated by quantitative reverse-transcription PCR. Results: Altogether, 117 significantly differentially expressed genes were detected at 48 h and 72 h (p < 0.05) in mitotane-treated samples relative to controls. Three significantly underexpressed genes involved in steroid hormone biosynthesis (HSD3B1, HSD3B2 and CYP21A2) and four significantly overexpressed genes (GDF15, ALDH1L2, TRIB3 and SERPINE2) have been validated. Conclusion: Gene-expression changes might be involved in the adrenal action of mitotane and in the inhibition of hormone secretion. Original submitted 20 January 2012; Revision submitted 17 May 2012.
Pubmed
Web of science
Création de la notice
26/10/2012 19:24
Dernière modification de la notice
20/08/2019 14:19
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