Cooperative binding of estrogen receptor to imperfect estrogen-responsive DNA elements correlates with their synergistic hormone-dependent enhancer activity.

Détails

ID Serval
serval:BIB_32F9C49EAA82
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Cooperative binding of estrogen receptor to imperfect estrogen-responsive DNA elements correlates with their synergistic hormone-dependent enhancer activity.
Périodique
EMBO Journal
Auteur(s)
Martinez E., Wahli W.
ISSN
0261-4189[print], 0261-4189[linking]
Statut éditorial
Publié
Date de publication
12/1989
Volume
8
Numéro
12
Pages
3781-3791
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
The Xenopus vitellogenin (vit) gene B1 estrogen-inducible enhancer is formed by two closely adjacent 13 bp imperfect palindromic estrogen-responsive elements (EREs), i.e. ERE-2 and ERE-1, having one and two base substitutions respectively, when compared to the perfect palindromic consensus ERE (GGTCANNNTGACC). Gene transfer experiments indicate that these degenerated elements, on their own, have a low or no regulatory capacity at all, but in vivo act together synergistically to confer high receptor- and hormone-dependent transcription activation to the heterologous HSV thymidine kinase promoter. Thus, the DNA region upstream of the vitB1 gene comprising these two imperfect EREs separated by 7 bp, was called the vitB1 estrogen-responsive unit (vitB1 ERU). Using in vitro protein-DNA interaction techniques, we demonstrate that estrogen receptor dimers bind cooperatively to the imperfect EREs of the vitB1 ERU. Binding of a first receptor dimer to the more conserved ERE-2 increases approximately 4- to 8-fold the binding affinity of the receptor to the adjacent less conserved ERE-1. Thus, we suggest that the observed synergistic estrogen-dependent transcription activation conferred by the pair of hormone-responsive DNA elements of the vit B1 ERU is the result of cooperative binding of two estrogen receptor dimers to these two adjacent imperfect EREs.
Mots-clé
Animals, Base Sequence, Binding, Competitive, DNA-Binding Proteins/metabolism, Deoxyribonuclease I/metabolism, Drug Synergism, Enhancer Elements, Genetic, Estrogens/genetics, Estrogens/metabolism, Hela Cells, Humans, Molecular Sequence Data, Receptors, Estrogen/metabolism, Transcription, Genetic, Transfection, Vitellogenins/genetics, Xenopus/genetics
Pubmed
Web of science
Création de la notice
24/01/2008 16:04
Dernière modification de la notice
20/08/2019 13:18
Données d'usage