Method for quantification of morphine and its 3- and 6- glucuronides, codeine, codeine glucuronide and 6-monoacetylmorphine in human blood by liquid chromatography-electrospray mass spectrometry for routine analysis in forensic toxicology.

Détails

ID Serval
serval:BIB_32A1B9BB94A7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Method for quantification of morphine and its 3- and 6- glucuronides, codeine, codeine glucuronide and 6-monoacetylmorphine in human blood by liquid chromatography-electrospray mass spectrometry for routine analysis in forensic toxicology.
Périodique
Journal of Chromatography. A
Auteur(s)
Dienes-Nagy A., Rivier L., Giroud C., Augsburger M., Mangin P.
ISSN
0021-9673
Statut éditorial
Publié
Date de publication
1999
Peer-reviewed
Oui
Volume
854
Numéro
1-2
Pages
109-118
Langue
anglais
Résumé
Simultaneous determination of opiates and their glucuronides in body fluids has a great practical interest in the forensic assessment of heroin intoxication. A selective and sensitive method for quantification of morphine and its 3- and 6-glucuronides, codeine, codeine glucuronide and 6-monoacetylmorphine (6-MAM) based on liquid chromatography-electrospray ionisation mass spectrometry is described. The drugs were analysed in human autopsy whole blood after solid-phase extraction on a C8 cartridge. The separation was performed on an ODS column in acetonitrile (analysis time 15 min). For the quantitative analysis, deuterated analogues of each compound were used as internal standards. Selected-ion monitoring was applied where the molecular ion was chosen for quantification. The limits of quantification were 0.5 ng/ml for morphine and 6-MAM and 1 ng/ml for the 6-glucuronide of morphine, codeine-6-glucuronide and codeine and 5 ng/ml for the 3-glucuronide of morphine.
Mots-clé
Calibration, Chromatography, Liquid, Codeine, Forensic Medicine, Glucuronides, Humans, Mass Spectrometry, Morphine
Pubmed
Web of science
Création de la notice
08/02/2008 18:43
Dernière modification de la notice
20/08/2019 14:18
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