Regulation of the peroxisome proliferator-activated receptor alpha gene by glucocorticoids.

Détails

ID Serval
serval:BIB_31B385E4571E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Regulation of the peroxisome proliferator-activated receptor alpha gene by glucocorticoids.
Périodique
Journal of Biological Chemistry
Auteur⸱e⸱s
Lemberger T., Staels B., Saladin R., Desvergne B., Auwerx J., Wahli W.
ISSN
0021-9258[print], 0021-9258[linking]
Statut éditorial
Publié
Date de publication
10/1994
Volume
269
Numéro
40
Pages
24527-24530
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
This study demonstrates that the expression of the peroxisome proliferator-activated receptor alpha (PPAR alpha) is regulated by glucocorticoid hormones in hepatocytes. Hydrocortisone, dexamethasone, and triamcinolone stimulated PPAR alpha mRNA synthesis in a dose-dependent manner in primary rat hepatocyte cultures. This glucocorticoid stimulation was inhibited by RU 486, a specific glucocorticoid antagonist. Moreover, in contrast to glucocorticoid hormones, the mineralocorticoid aldosterone had only a weak effect, suggesting that the hormonal stimulation of PPAR alpha was mediated by the glucocorticoid receptor. The induction was not prevented by cycloheximide treatment of the hepatocytes, indicating that it was mediated by preexisting glucocorticoid receptor. Finally, the RNA synthesis inhibitor actinomycin D abolished the stimulatory effect of dexamethasone, and nuclear run-on analysis showed an increase of PPAR alpha transcripts after hormonal induction. Thus, the PPAR alpha gene is an early response gene of glucocorticoids that control its expression at the transcriptional level.
Mots-clé
Animals, Base Sequence, Gene Expression Regulation/drug effects, Glucocorticoids/pharmacology, Male, Molecular Sequence Data, RNA, Messenger/analysis, Rats, Receptors, Cytoplasmic and Nuclear/genetics, Transcription Factors/genetics, Transcription, Genetic/drug effects
Pubmed
Web of science
Création de la notice
24/01/2008 15:27
Dernière modification de la notice
20/08/2019 13:17
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