Measurement of sympathetic nerve activity in the unanesthetized rat

Détails

ID Serval
serval:BIB_312A6FE29D8E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Measurement of sympathetic nerve activity in the unanesthetized rat
Périodique
Journal of Applied Physiology
Auteur(s)
Fluckiger  J. P., Gremaud  G., Waeber  B., Kulik  A., Ichino  A., Nussberger  J., Brunner  H. R.
ISSN
8750-7587 (Print)
Statut éditorial
Publié
Date de publication
07/1989
Volume
67
Numéro
1
Pages
250-5
Notes
Journal Article --- Old month value: Jul
Résumé
A new system was developed in our laboratory to continuously monitor intra-arterial pressure, heart rate, and sympathetic nerve activity in unanesthetized rats. The animals were prepared 24 h before the start of the experiments. Sympathoneural traffic was measured at the level of splanchnic nerve. The amplitude of the spikes recorded at this level was utilized to express sympathetic nerve activity. The amplitude of the residual electroneurogram signal present 30 min after the rats were killed was 32 +/- 2 mV (mean +/- SE; n = 11). For analysis, these background values were subtracted from values determined in vivo. The nerve we studied contains postganglionic fibers, since electrical activity decreased in response to ganglionic blockade with pentolinium (1.25 mg/min iv for 4 min). The amplitude of spikes fell by 43 +/- 4% (n = 4). Sympathetic nerve activity was highly reproducible at a 24-h interval (104 +/- 26 vs. 111 +/- 27 mV for the amplitude of spikes; n = 11). Dose-response curves to the alpha 1-stimulant methoxamine and to bradykinin were established in four rats. The increase in blood pressure induced by methoxamine caused a dose-dependent fall in sympathetic nerve activity, whereas the blood pressure reduction resulting from bradykinin was associated with a dose-dependent activation of sympathetic drive. These data therefore indicate that it is possible with out system to accurately measure sympathetic nerve activity in the awake rat, together with intra-arterial pressure and heart rate.
Mots-clé
Animals *Blood Pressure *Heart Rate Male Monitoring, Physiologic/*methods Rats Rats, Inbred Strains Splanchnic Nerves/physiology Sympathetic Nervous System/*physiology
Pubmed
Web of science
Création de la notice
05/03/2008 16:39
Dernière modification de la notice
20/08/2019 13:16
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