Secreted aspartic proteinase family of Candida tropicalis

Détails

ID Serval
serval:BIB_3107821ADB76
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Secreted aspartic proteinase family of Candida tropicalis
Périodique
Infection and Immunity
Auteur(s)
Zaugg  C., Borg-Von Zepelin  M., Reichard  U., Sanglard  D., Monod  M.
ISSN
0019-9567 (Print)
Statut éditorial
Publié
Date de publication
01/2001
Volume
69
Numéro
1
Pages
405-12
Notes
Journal Article --- Old month value: Jan
Résumé
Medically important yeasts of the genus Candida secrete aspartic proteinases (Saps), which are of particular interest as virulence factors. Like Candida albicans, Candida tropicalis secretes in vitro one dominant Sap (Sapt1p) in a medium containing bovine serum albumin (BSA) as the sole source of nitrogen. Using the gene SAPT1 as a probe and under low-stringency hybridization conditions, three new closely related gene sequences, SAPT2 to SAPT4, encoding secreted proteinases were cloned from a C. tropicalis lambdaEMBL3 genomic library. All bands identified by Southern blotting of EcoRI-digested C. tropicalis genomic DNA with SAPT1 could be assigned to a specific SAP gene. Therefore, the SAPT gene family of C. tropicalis is likely to contain only four members. Interestingly, the SAPT2 and SAPT3 gene products, Sapt2p and Sapt3p, which have not yet been detected in C. tropicalis cultures in vitro, were produced as active recombinant enzymes with the methylotrophic yeast Pichia pastoris as an expression system. As expected, reverse transcriptase PCR experiments revealed a strong SAPT1 signal with RNA extracted from cells grown in BSA medium. However, a weak signal was obtained with all other SAPT genes under several conditions tested, showing that these SAPT genes could be expressed at a basic level. Together, these experiments suggest that the gene products Sapt2p, Sapt3p, and Sapt4p could be produced under conditions yet to be described in vitro or during infection.
Mots-clé
Amino Acid Sequence Aspartic Endopeptidases/chemistry/*genetics/physiology Candida/*enzymology/genetics/pathogenicity Cloning, Molecular Molecular Sequence Data Recombinant Proteins/chemistry Reverse Transcriptase Polymerase Chain Reaction
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 16:47
Dernière modification de la notice
20/08/2019 13:15
Données d'usage