Identification of estrogen-responsive DNA sequences by transient expression experiments in a human breast cancer cell line.

Détails

ID Serval
serval:BIB_30D4EB5388F4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Identification of estrogen-responsive DNA sequences by transient expression experiments in a human breast cancer cell line.
Périodique
Nucleic Acids Research
Auteur(s)
Seiler-Tuyns A., Walker P., Martinez E., Mérillat A.M., Givel F., Wahli W.
ISSN
0305-1048[print], 0305-1048[linking]
Statut éditorial
Publié
Date de publication
11/1986
Volume
14
Numéro
22
Pages
8755-8770
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
The expression of a hybrid gene formed by the promoter region of the Xenopus laevis vitellogenin gene B1 and the CAT coding region is regulated by estrogen when the gene is transfected into hormone-responsive MCF-7 cells. Furthermore, the 5' flanking region of the gene B1 alone can confer inducibility to heterologous promoters, although to a varying extent depending on the promoter used. Deletion mapping of he vitellogenin hormone-responsive sequences revealed that a 13 bp element 5'-AGTCACTGTGACC-3' at position -334 is essential for estrogen inducibility. We have shown previously that this 13 bp element is present upstream of several liver-specific estrogen-inducible genes.
Mots-clé
Acetyltransferases/genetics, Animals, Breast Neoplasms/genetics, Breast Neoplasms/metabolism, Cell Line, Chloramphenicol O-Acetyltransferase, Chromosome Deletion, Estradiol/pharmacology, Female, Genes/drug effects, Globins/genetics, Humans, Mutation, Plasmids, Promoter Regions, Genetic, Transcription, Genetic/drug effects, Vitellogenins/genetics, Xenopus
Pubmed
Web of science
Création de la notice
24/01/2008 17:04
Dernière modification de la notice
20/08/2019 14:15
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