Gene transfer to urethral strictures in rabbits: a preliminary report.

Détails

ID Serval
serval:BIB_30B2CF81B0D4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Gene transfer to urethral strictures in rabbits: a preliminary report.
Périodique
Bju International
Auteur⸱e⸱s
Meria P., Anidjar M., Brouland J.P., Teillac P., Berthon P., Cussenot O.
ISSN
1464-4096 (Print)
ISSN-L
1464-4096
Statut éditorial
Publié
Date de publication
2000
Peer-reviewed
Oui
Volume
85
Numéro
9
Pages
1120-1125
Langue
anglais
Résumé
OBJECTIVE: To assess the rationale for virus-mediated gene transfer into the urethra in vivo and in vitro, using a rabbit model, as this is an attractive approach to prevent recurrence after the endoscopic management of urethral strictures.
MATERIALS AND METHODS: Primary cultures of rabbit urethral stromal cells were infected with adenoviral and retroviral solutions carrying a nucleus-targeted beta-galactosidase (beta-Gal) reporter gene (respectively 109 and 107 plaque-forming units/mL). In addition, to mimic the human clinical situation, a model was developed of thermally induced stricture in rabbit urethra which produced fibrotic stenosis within 15 days. Using a prototype channelled balloon catheter, these strictures were endoscopically dilated and then instilled with the beta-Gal adenoviral or retroviral constructs.
RESULTS: The application of recombinant adenovirus and retrovirus harbouring a nucleus-targeted beta-Gal reporter gene to cultured rabbit urethral stromal cells resulted in a high transduction efficiency of up to 90% and 96%, respectively. Five days after infection, histochemical and immunohistochemical staining of the strictured urethrae showed a 3% rate of transfection targeted to stromal cells within the fibrosis, confirmed by polymerase chain reaction (PCR) analysis. Adjacent and distal spread of the virus was excluded by histochemistry, immunohistochemistry and PCR.
CONCLUSION: These results represent the first report of endoscopic adenovirus and retrovirus-mediated gene transfer to the urethra. Although at a low rate, transduction reached stromal cells transmurally within the induced strictures and was site-specific.
Mots-clé
Adenoviridae/genetics, Animals, Cells, Cultured, Fibroblasts, Gene Transfer Techniques, Genetic Therapy/methods, Rabbits, Retroviridae/genetics, Stromal Cells, Transfection, Urethral Stricture/therapy
Pubmed
Web of science
Création de la notice
13/10/2015 9:29
Dernière modification de la notice
20/08/2019 13:15
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