Cytotoxic T cell clone-specific monoclonal antibodies used to select clonotypic antigen-specific cytotoxic T cells.

Détails

ID Serval
serval:BIB_309CE1853B96
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Cytotoxic T cell clone-specific monoclonal antibodies used to select clonotypic antigen-specific cytotoxic T cells.
Périodique
European Journal of Immunology
Auteur⸱e⸱s
Acha-Orbea H., Zinkernagel R.M., Hengartner H.
ISSN
0014-2980 (Print)
ISSN-L
0014-2980
Statut éditorial
Publié
Date de publication
1985
Volume
15
Numéro
1
Pages
31-36
Langue
anglais
Résumé
Two rat monoclonal antibodies (mAb) have been produced which recognize a clone-specific determinant on the alloreactive cytotoxic T lymphocyte (CTL) clone 3 F9. CTL clone 3F9 of BALB/c origin is specific for H-2Db and can be grown by weekly restimulation with irradiated stimulator spleen cells expressing H-2Db in the presence of interleukin 2. Two mAb against T cell clone 3F9, 44-22-1(IgG2a) and 46-6 B5(IgM), have been proven to be clone specific: they inhibit cytotoxic activity of 3F9 only and bind specifically to 3 F9 when compared in a panel of different CTL clones, or cells from different mixed lymphocyte cultures (MLC), BALB/c thymus and spleen cells. The mAb 44-22-1 has been used to sort cells from a primary MLC BALB/c anti-H-2Db by fluorescence-activated cell sorter (FACS) to select CTL expressing 3 F9 clonotype-specific determinants. The lymphocytes reactive with 44-22-1 represent a minor subpopulation of the CTL of the primary MLC. The specific alloreactive cytotoxicity of unsorted lymphocytes of the bulk primary MLC could not be inhibited by the mAb 44-22-1 and 46-6 B5 whereas the sorted 3 F9 clonotype-positive cultures could be inhibited very effectively. All the CTL clones derived from the FACS-sorted clonotype-positive culture show all the same properties and are identical with clone 3 F9 with respect to antigen-specific cytotoxicity, inhibition of cytotoxicity by the mAb and surface markers.
Mots-clé
Animals, Antibodies, Monoclonal/analysis, Antibodies, Monoclonal/biosynthesis, Binding, Competitive, Cell Separation, Clone Cells/immunology, Epitopes/immunology, Lymphocyte Activation, Lymphocyte Culture Test, Mixed, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Rats, Rats, Inbred Strains, T-Lymphocytes, Cytotoxic/immunology
Pubmed
Web of science
Création de la notice
24/01/2008 15:48
Dernière modification de la notice
20/08/2019 14:15
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