Protein kinase C-activating tumor promoters enhance the differentiation of astrocytes in aggregating fetal brain cell cultures.

Détails

ID Serval
serval:BIB_2FF769AD045E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Protein kinase C-activating tumor promoters enhance the differentiation of astrocytes in aggregating fetal brain cell cultures.
Périodique
Journal of Neurochemistry
Auteur(s)
Honegger P.
ISSN
0022-3042 (Print)
ISSN-L
0022-3042
Statut éditorial
Publié
Date de publication
1986
Volume
46
Numéro
5
Pages
1561-1566
Langue
anglais
Résumé
Serum-free aggregating cell cultures of fetal rat telencephalon treated with the potent tumor promoter phorbol 12-myristate 13-acetate (PMA) showed a marked, rapid, and sustained increase in the activity of the astrocyte-specific enzyme glutamine synthetase (GS). This effect was accompanied by a small increase in RNA synthesis and a progressive reduction in DNA synthesis. Only mitotically active cultures were responsive to PMA treatments. Since in aggregate cultures astrocytes are the preponderant cell type, both in number and mitotic activity, it can be concluded that PMA induces and/or enhances the terminal differentiation of astrocytes. The developmental expression of GS was also greatly stimulated by mezerein, a potent nonphorbol tumor promoter, but not by 4 alpha-phorbol 12,13-didecanoate, a nonpromoting phorbol ester. Since both tumor promoters, PMA and mezerein, are potent and specific activators of C-kinase, it is suggested that C-kinase plays a regulatory role in the growth and differentiation of normal astrocytes.
Mots-clé
Animals, Astrocytes/cytology, Brain/cytology, Carcinogens/pharmacology, Cell Aggregation, Cell Differentiation/drug effects, Cells, Cultured, Diterpenes, Embryo, Mammalian, Enzyme Activation/drug effects, Epidermal Growth Factor/pharmacology, Glutamate-Ammonia Ligase/metabolism, Protein Kinase C/metabolism, Rats, Rats, Inbred Strains, Telencephalon/cytology, Terpenes/pharmacology, Tetradecanoylphorbol Acetate/pharmacology, Time Factors
Pubmed
Web of science
Création de la notice
24/01/2008 13:12
Dernière modification de la notice
20/08/2019 13:14
Données d'usage