Digital expression profiles of human endogenous retroviral families in normal and cancerous tissues.

Détails

ID Serval
serval:BIB_2FE0266F92B3
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Digital expression profiles of human endogenous retroviral families in normal and cancerous tissues.
Périodique
Cancer Immunity
Auteur⸱e⸱s
Stauffer Y., Theiler G., Sperisen P., Lebedev Y., Jongeneel C.V.
ISSN
1424-9634 (Electronic)
ISSN-L
1424-9634
Statut éditorial
Publié
Date de publication
2004
Volume
4
Pages
2
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Résumé
Human endogenous retroviruses (HERVs) are remnants of ancient retroviral infections that became fixed in the germ line DNA millions of years ago. The fact that humoral and cellular immune responses against HERV-encoded proteins have been identified in cancer patients suggests that these antigens might be used in cancer immunotherapy or diagnosis. We analyzed the digital expression patterns of the HERV-K (HML-2), -W, -H and -E families in normal and cancerous tissues. Thirty-one proviral members of the HERV-K family and one representative each for the other HERV families were used as probes to search human EST data. Matching of HERV proviruses to ESTs was HERV family-specific and the expression profiles of the HERV families distinct. The HERV-K family was expressed in normal tissues such as muscle, skin and brain, as well as in germ cell tumors and other cancerous tissues. HERV-H was the only family expressed in cancers of the intestine, bone marrow, bladder and cervix, and was more highly expressed than the other families in cancers of the stomach, colon and prostate. In contrast, HERV-W was predominantly expressed in normal placenta. Expression patterns were confirmed by MPSS (massively parallel signature sequencing) data where available. For the HERV-K family, we mapped most ESTs to their corresponding proviruses and assessed the coding capacities of the matched proviruses. This study shows that HERV families are more widely expressed than originally thought and that some members of the HERV-K and -H families could encode targets for cancer immunotherapy.
Mots-clé
Chromosome Mapping, Cluster Analysis, DNA Probes/genetics, DNA, Viral/genetics, Endogenous Retroviruses/genetics, Evolution, Molecular, Expressed Sequence Tags, Female, Gene Expression Profiling/methods, Gene Expression Profiling/statistics & numerical data, Genes, Viral/genetics, Genome, Human, Genome, Viral, Humans, Male, Neoplasms/genetics, Neoplasms/virology, Oligonucleotide Array Sequence Analysis/methods, Oligonucleotide Array Sequence Analysis/statistics & numerical data, Open Reading Frames/genetics, Organ Specificity/genetics, Proviruses/genetics, Species Specificity, Viral Structural Proteins/genetics
Pubmed
Création de la notice
24/01/2008 15:39
Dernière modification de la notice
20/08/2019 13:14
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