Complexity, polymorphism, and connectivity of mouse Vk gene families

Détails

ID Serval
serval:BIB_2F5370230626
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Complexity, polymorphism, and connectivity of mouse Vk gene families
Périodique
Immunogenetics
Auteur(s)
Kofler  R., Duchosal  M. A., Dixon  F. J.
ISSN
0093-7711 (Print)
Statut éditorial
Publié
Date de publication
1989
Volume
29
Numéro
2
Pages
65-74
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.
Résumé
To define the polymorphism and extent of the mouse immunoglobulin kappa (Igk) gene complex, we have analyzed restriction-enzyme digested genomic DNA from 33 inbred strains of mice with labeled DNA probes corresponding to 16 Vk protein groups (1 of them previously undescribed) and the Jk/Ck region (V, variable; J, joining; C, constant). These probes detected between 1 and 25 distinct restriction enzyme fragments (REF) that appeared in up to eight polymorphic patterns, thus defining eight mouse Igk haplotypes. The investigated portion of the Vk repertoire was estimated to encompass between 60 and 120 discernable Vk gene-containing REFs. In contrast to mouse VH gene families, several Vk gene families defined by these probes appeared to overlap. This observation has implications for Vk gene analyses by nucleic acid hybridization and raises the possibility that the Vk gene complex is a continuum of related sequences.
Mots-clé
Amino Acid Sequence Animals Base Sequence *Genes, Immunoglobulin Haplotypes Immunoglobulin Variable Region/*genetics Immunoglobulin kappa-Chains/*genetics Mice Mice, Inbred Strains/genetics Molecular Sequence Data Multigene Family Polymorphism, Restriction Fragment Length Sequence Homology, Nucleic Acid
Pubmed
Web of science
Création de la notice
25/01/2008 15:24
Dernière modification de la notice
20/08/2019 13:13
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