Beta 3-adrenoceptor knockout in C57BL/6J mice depresses the occurrence of brown adipocytes in white fat.

Détails

ID Serval
serval:BIB_2ED4AAA7CF84
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Beta 3-adrenoceptor knockout in C57BL/6J mice depresses the occurrence of brown adipocytes in white fat.
Périodique
European Journal of Biochemistry
Auteur(s)
Jimenez M., Barbatelli G., Allevi R., Cinti S., Seydoux J., Giacobino J.P., Muzzin P., Preitner F.
ISSN
0014-2956 (Print)
ISSN-L
0014-2956
Statut éditorial
Publié
Date de publication
2003
Volume
270
Numéro
4
Pages
699-705
Langue
anglais
Résumé
White and brown adipocytes are usually located in distinct depots; however, in response to cold, brown adipocytes appear in white fat. This response is mediated by beta-adrenoceptors but there is a controversy about the subtype(s) involved. In the present study, we exposed to cold beta 3-adrenoceptor knockout mice (beta 3KO) on a C57BL/6J genetic background and measured in white adipose tissue the density of multilocular cells and the expression of the brown adipocyte marker uncoupling protein-1 (UCP1). In brown fat of beta 3KO mice, UCP1 expression levels were normal at 24 degrees C as well as after a 10-day cold exposure. Strikingly, under both conditions, in the white fat of beta 3KO mice the levels of UCP1 mRNA and protein as well as the density of multilocular cells were decreased. These results indicate that beta 3-adrenoceptors play a major role in the appearance of brown adipocytes in white fat and suggest that the brown adipocytes present in white fat differ from those in brown fat.
Mots-clé
Adipocytes/metabolism, Adipose Tissue, Brown/metabolism, Animals, Blotting, Northern, Blotting, Western, Carrier Proteins/genetics, Carrier Proteins/metabolism, Electron Transport Complex IV/metabolism, Female, Gene Expression Regulation, Immunoenzyme Techniques, Ion Channels, Male, Membrane Proteins/genetics, Membrane Proteins/metabolism, Mice, Mice, Inbred C57BL, Mice, Knockout, Mitochondria/metabolism, Mitochondrial Proteins, RNA, Messenger/metabolism, Receptors, Adrenergic, beta-3/physiology, Uncoupling Agents/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
18/12/2012 15:50
Dernière modification de la notice
20/08/2019 13:13
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