Descemet-stripping automated endothelial keratoplasty: effect of anterior lamellar corneal tissue-on/-off storage condition on Descemet-stripping automated endothelial keratoplasty donor tissue.

Détails

ID Serval
serval:BIB_2EC00F5B1E77
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Descemet-stripping automated endothelial keratoplasty: effect of anterior lamellar corneal tissue-on/-off storage condition on Descemet-stripping automated endothelial keratoplasty donor tissue.
Périodique
Cornea
Auteur⸱e⸱s
Ide T., Yoo S.H., Kymionis G.D., Goldman J.M., Perez V.L., O'Brien T.P.
ISSN
1536-4798 (Electronic)
ISSN-L
0277-3740
Statut éditorial
Publié
Date de publication
08/2008
Peer-reviewed
Oui
Volume
27
Numéro
7
Pages
754-757
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
To compare the effect of storing Descemet-stripping automated endothelial keratoplasty (DSAEK) tissue with anterior lamellar corneal tissue (ALCT)-on versus -off.
An in vitro model was used with corneoscleral rims and DSAEK quality paired corneas. After microkeratome-assisted excision of ALCT, 4 pairs of corneas (8 eyes) were stored with the ALCT left on the stroma (on) and the others with ALCT off the stroma (off) for 24 hours in Optisol GS solution. A vital dye assay was used to identify devitalized and necrotic endothelial cells with alizarin red S and trypan blue.
Corneal endothelial cell damage was observed in the ALCT-off specimens, whereas almost no staining was observed in the ALCT-on samples. In addition, the ALCT-off donor corneas were clinically edematous and opaque, whereas the ALCT-on corneas were clear. Moreover, Descemet membranes of ALCT-off samples were found to be loose and easily detached from the stroma, with many Descemet striae observed in the specimens.
This study shows that endothelial damage occurs in ALCT-off corneas. We hypothesize that the absence of the Bowman layer may contribute to the damage, because it has been shown that the Bowman layer provides a barrier function. These data suggest that it is important to keep the ALCT/Bowman layer on the stromal side of the DSAEK graft as long as possible to avoid stromal swelling and endothelial damage.
Mots-clé
Adult, Aged, Anthraquinones/pharmacology, Cell Count, Cell Survival, Chondroitin Sulfates, Coloring Agents/pharmacology, Complex Mixtures, Cornea, Corneal Transplantation/pathology, Cryopreservation, Culture Media, Serum-Free, Descemet Membrane/surgery, Dextrans, Endothelium, Corneal/drug effects, Endothelium, Corneal/pathology, Endothelium, Corneal/transplantation, Gentamicins, Humans, Middle Aged, Organ Preservation, Tissue Donors, Trypan Blue/pharmacology
Pubmed
Web of science
Création de la notice
02/10/2019 8:50
Dernière modification de la notice
06/10/2019 5:26
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