Beta subunits of the olfactory cyclic nucleotide-gated channel form a nitric oxide activated Ca2+ channel

Détails

ID Serval
serval:BIB_2E5A44447111
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Beta subunits of the olfactory cyclic nucleotide-gated channel form a nitric oxide activated Ca2+ channel
Périodique
Neuron
Auteur⸱e⸱s
Broillet  M. C., Firestein  S.
ISSN
0896-6273 (Print)
Statut éditorial
Publié
Date de publication
06/1997
Volume
18
Numéro
6
Pages
951-8
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Jun
Résumé
Cyclic nucleotide-gated channels are important in visual and olfactory transduction, and possibly in other neuronal functions. These channels have a complex permeability to Ca2+ ions that may be important in their cellular functions. They are composed of two different subunits, alpha and beta, that have been cloned and expressed, but the beta subunit alone cannot be activated by cyclic nucleotides, confounding the analysis of its characteristics. However, we found that nitric oxide can activate the homomeric expressed beta subunit, and the resulting channel possesses many properties of the L-type Ca2+ channels, including high permeability to Ca2+ ions and sensitivity to Ca2+ channel blockers. Thus, the Ca2+ permeability characteristics of native channels are mostly conferred by properties of the beta subunit, and the beta subunit alone can act as a NO-sensitive Ca2+ channel. A nearly identical conductance activated by NO is present in the membrane of rat vomeronasal neurons, indicating that homomeric beta channels exist in vivo.
Mots-clé
Animals Calcium Channels/*physiology Cell Line Cysteine/analogs & derivatives/pharmacology Electric Conductivity Humans Hydrogen-Ion Concentration Ion Channel Gating Nitric Oxide/*physiology Nucleotides, Cyclic/*physiology Rats Rats, Sprague-Dawley Receptors, Odorant/*physiology *S-Nitrosothiols
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 11:01
Dernière modification de la notice
20/08/2019 13:12
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