Loss of endoplasmic reticulum calcium pump expression in choroid plexus tumours.

Détails

ID Serval
serval:BIB_2DDEC976D599
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Loss of endoplasmic reticulum calcium pump expression in choroid plexus tumours.
Périodique
Neuropathology and Applied Neurobiology
Auteur⸱e⸱s
Ait-Ghezali L., Arbabian A., Jeibmann A., Hasselblatt M., Hallaert G.G., Van den Broecke C., Gray F., Brouland J.P., Varin-Blank N., Papp B.
ISSN
1365-2990 (Electronic)
ISSN-L
0305-1846
Statut éditorial
Publié
Date de publication
2014
Peer-reviewed
Oui
Volume
40
Numéro
6
Pages
726-735
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
AIMS: Sarco/Endoplasmic Reticulum Calcium ATPase-type calcium pumps (SERCA enzymes) control cell activation by sequestering calcium ions from the cytosol into the endoplasmic reticulum. Although endoplasmic reticulum calcium signalling plays an important role in the regulation of choroid plexus epithelial function, SERCA expression in the choroid plexus has not been investigated so far.
METHODS: In this work we investigated the expression of the SERCA3-type calcium pump in choroid plexus epithelial cells grown in vitro, and in normal and hyperplastic choroid plexus tissue, in choroid plexus papillomas displaying various degrees of atypia, and in choroid plexus carcinoma by immunohistochemistry in situ.
RESULTS: Whereas normal choroid plexus epithelial cells express SERCA3 abundantly, SERCA3 expression is strongly decreased in papillomas, and is absent in choroid plexus carcinoma, while expression in hyperplastic epithelium is high, similarly to normal epithelium. SERCA3 expression was detected also in normal primary choroid plexus epithelial cells grown in vitro, and expression was markedly enhanced by short-chain fatty acid-type cell differentiation inducing agents, including valproate.
CONCLUSION: These observations show that SERCA3 is a new phenotypic marker of normal choroid plexus epithelial differentiation, and that SERCA3 constitutes an early tumour marker 'by loss of expression' in the choroid plexus that may be useful to distinguish hyperplastic processes from papillomas. Endoplasmic reticulum calcium homeostasis becomes anomalous, due to loss of SERCA3 expression, already in benign neoplastic lesions of the choroid plexus epithelium.
Mots-clé
Choroid Plexus/metabolism, Choroid Plexus Neoplasms/metabolism, Epithelial Cells/metabolism, Humans, Papilloma, Choroid Plexus/metabolism, Primary Cell Culture, Sarcoplasmic Reticulum Calcium-Transporting ATPases/metabolism
Pubmed
Web of science
Création de la notice
13/10/2015 10:19
Dernière modification de la notice
20/08/2019 13:12
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