Waveform modulation of negative-pressure wound therapy in the murine model.

Détails

ID Serval
serval:BIB_2DB8372D8DA0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Waveform modulation of negative-pressure wound therapy in the murine model.
Périodique
Plastic and reconstructive surgery
Auteur(s)
Dastouri P., Helm D.L., Scherer S.S., Pietramaggiori G., Younan G., Orgill D.P.
ISSN
1529-4242 (Electronic)
ISSN-L
0032-1052
Statut éditorial
Publié
Date de publication
04/2011
Peer-reviewed
Oui
Volume
127
Numéro
4
Pages
1460-1466
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Negative-pressure wound therapy applied with a porous foam interface has been shown to accelerate granulation-tissue formation when a cyclic application mode of suction is applied, but the optimal waveform has not been determined. The authors hypothesized that changes in the suction waveform applied to wounds would modulate the biological response of granulation tissue formation.
A vacuum-assisted closure device (Kinetic Concepts, Inc., San Antonio, Texas) was applied to full-thickness wounds in 48 male diabetic mice (C57BL/KsJ-Lepr db), which were treated with six different waveforms: square waveforms of 125 mmHg of suction for 2 minutes, alternating with 50 mmHg of suction for 2 minutes, 5 minutes, or 10 minutes; triangular waveform with a 7-minute period oscillating between 50 and 125 mmHg; and static suction at 125 mmHg or static suction at 0 mmHg (occlusive dressing). Wounds were quantitatively evaluated for granulation tissue thickness as well as the number of proliferating cells and the number of blood vessels of the newly formed granulation tissue.
At 7 days, the continuous and triangular waveforms induced the thickest granulation tissue, with high rates of cellular proliferation and blood vessel counts compared with square wave and occlusive dressing control wounds. Decreasing square waveform frequency significantly increased granulation tissue thickness, cellular proliferation, and blood vessel counts.
Waveform modulation has a significant effect on granulation tissue formation, angiogenesis, and cellular proliferation in excisional wounds in diabetic mice. The rapid change in pressure seen in our square wave model may be detrimental to granulation tissue formation.

Mots-clé
Animals, Cell Proliferation, Diabetes Mellitus, Experimental/physiopathology, Granulation Tissue/pathology, Granulation Tissue/physiology, Ki-67 Antigen/analysis, Male, Mice, Mice, Inbred C57BL, Negative-Pressure Wound Therapy/methods, Neovascularization, Physiologic, Platelet Endothelial Cell Adhesion Molecule-1/analysis, Skin/blood supply, Skin/injuries, Suction/methods, Wound Healing/physiology
Pubmed
Web of science
Création de la notice
16/01/2018 16:10
Dernière modification de la notice
20/08/2019 14:12
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