CIITA is a transcriptional coactivator that is recruited to MHC class II promoters by multiple synergistic interactions with an enhanceosome complex.

Détails

ID Serval
serval:BIB_2DA42E62A0B7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
CIITA is a transcriptional coactivator that is recruited to MHC class II promoters by multiple synergistic interactions with an enhanceosome complex.
Périodique
Genes & development
Auteur(s)
Masternak K, Muhlethaler-Mottet A, Villard J, Zufferey M, Steimle V, Reith W
Statut éditorial
Publié
Date de publication
05/2000
Peer-reviewed
Oui
Langue
anglais
Résumé
By virtue of its control over major histocompatibility complex class II (MHC-II) gene expression, CIITA represents a key molecule in the regulation of adaptive immune responses. It was first identified as a factor that is defective in MHC-II deficiency, a hereditary disease characterized by the absence of MHC-II expression. CIITA is a highly regulated transactivator that governs all spatial, temporal, and quantitative aspects of MHC-II expression. It has been proposed to act as a non-DNA-binding transcriptional coactivator, but evidence that it actually functions at the level of MHC-II promoters was lacking. By means of chromatin immunoprecipitation assays, we show here for the first time that CIITA is physically associated with MHC-II, as well as HLA-DM, Ii, MHC-I, and beta(2)m promoters in vivo. To dissect the mechanism by which CIITA is recruited to the promoter, we have developed a DNA-dependent coimmunoprecipitation assay and a pull-down assay using immobilized promoter templates. We demonstrate that CIITA recruitment depends on multiple, synergistic protein-protein interactions with DNA-bound factors constituting the MHC-II enhanceosome. CIITA therefore represents a paradigm for a novel type of regulatory and gene-specific transcriptional cofactor.
Mots-clé
MHC class II, CIITA, enhanceosome, coactivator, transcription, gene regulation
Pubmed
Open Access
Oui
Création de la notice
23/03/2020 12:44
Dernière modification de la notice
25/03/2020 7:26
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