Biophysical characterization of the interaction of endotoxins with hemoglobins.

Détails

ID Serval
serval:BIB_2D85E6DF72F7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Biophysical characterization of the interaction of endotoxins with hemoglobins.
Périodique
Medicinal chemistry
Auteur⸱e⸱s
Howe J., Hammer M., Alexander C., Rössle M., Fournier K., Mach J.P., Waelli T., Gorczynski R.M., Ulmer A.J., Zähringer U., Rietschel E.T., Brandenburg K.
ISSN
1573-4064
Statut éditorial
Publié
Date de publication
2007
Peer-reviewed
Oui
Volume
3
Numéro
1
Pages
13-20
Langue
anglais
Notes
Publication types: In Vitro ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Bacterial endotoxin (lipopolysaccharide, LPS) is the major component of the outer leaflet of the outer membrane in gram-negative bacteria. During severe infections, bacteria may reach the blood circuit of humans, and endotoxins may be released from the bacteria due to cell division or cell death. In particular enterobacterial forms of LPS represent extremely strong activator molecules of the human immune system causing a rapid induction of cytokine production in monocytes and macrophages. Various mammalian blood proteins have been documented to display LPS binding activities mediating normally decreasing effects in the biological activity of LPS. In more recent studies, the essential systemic oxygen transportation protein hemoglobin (Hb) has been shown to amplify LPS-induced cytokine production on immune cells. The mechanism responsible for this effect is poorly understood. Here, we characterize the interaction of hemoglobin with LPS by using biophysical methods. The data presented, revealing the changes of the type and size of supramolecular aggregates of LPS in the presence of Hb, allow a better understanding of the hemoglobin-induced increase in bioactivity of LPS.
Mots-clé
Animals, Biophysical Phenomena, Biophysics, Calorimetry, Crystallography, X-Ray, Cytokines/blood, Endotoxins/chemistry, Hemoglobins/chemistry, Humans, Lasers, Lipid A/chemistry, Lipopolysaccharides/chemistry, Molecular Conformation, Molecular Weight, Monocytes/drug effects, Monocytes/metabolism, Salmonella enterica/chemistry, Scattering, Radiation, Sheep, Spectroscopy, Fourier Transform Infrared
Pubmed
Web of science
Création de la notice
18/11/2009 9:45
Dernière modification de la notice
20/08/2019 13:12
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