Differential gene expression in response to mechanical wounding and insect feeding in Arabidopsis.

Détails

ID Serval
serval:BIB_2D6AEF9C5F6F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Differential gene expression in response to mechanical wounding and insect feeding in Arabidopsis.
Périodique
Plant Cell
Auteur⸱e⸱s
Reymond P., Weber H., Damond M., Farmer E.E.
ISSN
1040-4651 (Print)
ISSN-L
1040-4651
Statut éditorial
Publié
Date de publication
2000
Volume
12
Numéro
5
Pages
707-720
Langue
anglais
Résumé
Wounding in multicellular eukaryotes results in marked changes in gene expression that contribute to tissue defense and repair. Using a cDNA microarray technique, we analyzed the timing, dynamics, and regulation of the expression of 150 genes in mechanically wounded leaves of Arabidopsis. Temporal accumulation of a group of transcripts was correlated with the appearance of oxylipin signals of the jasmonate family. Analysis of the coronatine-insensitive coi1-1 Arabidopsis mutant that is also insensitive to jasmonate allowed us to identify a large number of COI1-dependent and COI1-independent wound-inducible genes. Water stress was found to contribute to the regulation of an unexpectedly large fraction of these genes. Comparing the results of mechanical wounding with damage by feeding larvae of the cabbage butterfly (Pieris rapae) resulted in very different transcript profiles. One gene was specifically induced by insect feeding but not by wounding; moreover, there was a relative lack of water stress-induced gene expression during insect feeding. These results help reveal a feeding strategy of P. rapae that may minimize the activation of a subset of water stress-inducible, defense-related genes.
Mots-clé
Animals, Arabidopsis/genetics, Arabidopsis/parasitology, DNA, Complementary, Gene Expression Regulation, Plant, Insects/physiology, RNA, Messenger/genetics, Signal Transduction
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 20:05
Dernière modification de la notice
20/08/2019 13:12
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