Signaling Lymphocytic Activation Molecule Family Member 1 Engagement Inhibits T Cell-B Cell Interaction and Diminishes Interleukin-6 Production and Plasmablast Differentiation in Systemic Lupus Erythematosus.

Détails

ID Serval
serval:BIB_2CF54215DED7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Signaling Lymphocytic Activation Molecule Family Member 1 Engagement Inhibits T Cell-B Cell Interaction and Diminishes Interleukin-6 Production and Plasmablast Differentiation in Systemic Lupus Erythematosus.
Périodique
Arthritis & rheumatology
Auteur(s)
Karampetsou M.P., Comte D., Suárez-Fueyo A., Katsuyama E., Yoshida N., Kono M., Kyttaris V.C., Tsokos G.C.
ISSN
2326-5205 (Electronic)
ISSN-L
2326-5191
Statut éditorial
Publié
Date de publication
01/2019
Peer-reviewed
Oui
Volume
71
Numéro
1
Pages
99-108
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Signaling lymphocytic activation molecule family member 1 (SLAMF1) homophilic interactions promote immunoglobulin production and T cell-B cell cross-talk. SLAMF1 is overexpressed on T and B cells in patients with systemic lupus erythematosus (SLE). This study was undertaken to determine the role of SLAMF1 monoclonal antibody (mAb) in modulating T cell-B cell interaction and B cell activation.
Anti-IgM-prestimulated naive or total B cells from either healthy donors or patients with SLE were cocultured with autologous T cells under CD3/CD28 stimulation, in the presence or absence of the SLAMF1 mAb. Naive B cells were stimulated with anti-IgM and CD40L in the presence of the SLAMF1 antibody. Cytokine production by CD4+ T cells and B cells was examined by flow cytometry and/or quantitative polymerase chain reaction. Plasmablast formation and T cell and B cell conjugates were assessed by flow cytometry. IgG and antinuclear antibody production was determined by enzyme-linked immunosorbent assay.
SLAMF1 ligation in a human peripheral blood T cell-B cell culture system reduced the following in both healthy controls and patients with SLE: conjugate formation, interleukin-6 (IL-6) production by B cells, IL-21 and IL-17A production by T cells, and Ig and autoantibody production. Whereas the SLAMF1 mAb directly affected the function of isolated peripheral B cells by decreasing IL-6 and Ig production in vitro, it did not affect cytokine production by isolated T cells stimulated in vitro.
The SLAMF1 antibody inhibits T cell-B cell interaction and suppresses B cell cytokine production and differentiation, thereby acting as a potential therapeutic tool in the treatment of patients with SLE.
Mots-clé
Adult, B-Lymphocytes/drug effects, B-Lymphocytes/immunology, Case-Control Studies, Coculture Techniques, Female, Humans, Interleukin-17/immunology, Interleukin-6/immunology, Interleukins/immunology, Lupus Erythematosus, Systemic/immunology, Lymphopoiesis/drug effects, Lymphopoiesis/immunology, Male, Middle Aged, Plasma Cells/cytology, Plasma Cells/drug effects, Plasma Cells/immunology, Signaling Lymphocytic Activation Molecule Family Member 1/antagonists & inhibitors, Signaling Lymphocytic Activation Molecule Family Member 1/immunology, T-Lymphocytes/drug effects, T-Lymphocytes/immunology, Th17 Cells/drug effects, Th17 Cells/immunology
Pubmed
Web of science
Open Access
Oui
Création de la notice
22/08/2018 8:08
Dernière modification de la notice
05/11/2019 6:10
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