Plasmodium falciparum merozoite surface protein 2: epitope mapping and fine specificity of human antibody response against non-polymorphic domains.

Détails

Ressource 1Télécharger: BIB_2CED1904C6D2.P001.pdf (1756.02 [Ko])
Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_2CED1904C6D2
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Plasmodium falciparum merozoite surface protein 2: epitope mapping and fine specificity of human antibody response against non-polymorphic domains.
Périodique
Malaria Journal
Auteur(s)
Balam S., Olugbile S., Servis C., Diakité M., D'Alessandro A., Frank G., Moret R., Nebie I., Tanner M., Felger I., Smith T., Kajava A.V., Spertini F., Corradin G.
ISSN
1475-2875 (Electronic)
ISSN-L
1475-2875
Statut éditorial
Publié
Date de publication
2014
Volume
13
Pages
510
Langue
anglais
Résumé
BACKGROUND: Two long synthetic peptides representing the dimorphic and constant C-terminal domains of the two allelic families of Plasmodium falciparum merozoite surface proteins 2 are considered promising malaria vaccine candidates. The aim of the current study is to characterize the immune response (epitope mapping) in naturally exposed individuals and relate immune responses to the risk of clinical malaria.
METHODS: To optimize their construction, the fine specificity of human serum antibodies from donors of different age, sex and living in four distinct endemic regions was determined in ELISA by using overlapping 20 mer peptides covering the two domains. Immune purified antibodies were used in Western blot and immunofluorescence assay to recognize native parasite derivate proteins.
RESULTS: Immunodominant epitopes were characterized, and their distribution was similar irrespective of geographic origin, age group and gender. Acquisition of a 3D7 family and constant region-specific immune response and antibody avidity maturation occur early in life while a longer period is needed for the corresponding FC27 family response. In addition, the antibody response to individual epitopes within the 3D7 family-specific region contributes to protection from malaria infection with different statistical weight. It is also illustrated that affinity-purified antibodies against the dimorphic or constant regions recognized homologous and heterologous parasites in immunofluorescence and homologous and heterologous MSP2 and other polypeptides in Western blot.
CONCLUSION: Data from this current study may contribute to a development of MSP2 vaccine candidates based on conserved and dimorphic regions thus bypassing the complexity of vaccine development related to the polymorphism of full-length MSP2.
Mots-clé
Plasmodium falciparum, MSP2, Dimorphic regions, C-terminal region, Epitope mapping, Fine specificity
Pubmed
Web of science
Open Access
Oui
Création de la notice
21/01/2015 11:03
Dernière modification de la notice
20/08/2019 13:11
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