Release of extracellular particles by recombinant vaccinia virus over-expressing the major envelope protein p37K.

Détails

ID Serval
serval:BIB_2CCFE9DF8607
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Release of extracellular particles by recombinant vaccinia virus over-expressing the major envelope protein p37K.
Périodique
Journal of General Virology
Auteur(s)
Schmutz C., Wittek R.
ISSN
0022-1317[print], 0022-1317[linking]
Statut éditorial
Publié
Date de publication
12/1995
Volume
76 ( Pt 12)
Pages
2963-2968
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
During the replication cycle of vaccinia virus, four different forms of viral particles are produced. The two extracellular enveloped forms, cell-associated enveloped virus and extracellular enveloped virus, are responsible for cell-to-cell transmission and long-range spread of infection both in vivo and in vitro. Despite the biological importance of the enveloped forms, the mechanism of envelopment and the components involved in this process have been analysed only recently. Therefore the individual steps and the rate-limiting factors of the envelopment process are still unknown. The protein p37K, an unglycosylated but acylated envelope protein of molecular mass 37 kDa, has been shown to be essential for envelopment. However, this study shows that over-expression of p37K by vaccinia virus recombinants reduces rather than increases the yield of infectious enveloped virus which is mainly due to the enveloped virions exhibiting a strongly diminished specific infectivity.
Mots-clé
Animals, Base Sequence, Cell Line, Cercopithecus aethiops, DNA, Recombinant, Humans, Membrane Proteins/biosynthesis, Molecular Sequence Data, Rabbits, Vaccinia virus/metabolism, Vaccinia virus/pathogenicity, Viral Envelope Proteins/biosynthesis, Viral Proteins/analysis, Virion/chemistry, Virion/physiology, Virus Replication/physiology
Pubmed
Web of science
Création de la notice
24/01/2008 10:43
Dernière modification de la notice
20/08/2019 13:11
Données d'usage