Early interleukin 12 production by macrophages in response to mycobacterial infection depends on interferon gamma and tumor necrosis factor alpha.

Détails

ID Serval
serval:BIB_2C851EA53E9F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Early interleukin 12 production by macrophages in response to mycobacterial infection depends on interferon gamma and tumor necrosis factor alpha.
Périodique
Journal of Experimental Medicine
Auteur⸱e⸱s
Flesch I.E., Hess J.H., Huang S., Aguet M., Rothe J., Bluethmann H., Kaufmann S.H.
ISSN
0022-1007 (Print)
ISSN-L
0022-1007
Statut éditorial
Publié
Date de publication
1995
Volume
181
Numéro
5
Pages
1615-1621
Langue
anglais
Résumé
Interleukin 12 (IL-12) produced by macrophages immediately after infection is considered essential for activation of a protective immune response against intracellular pathogens. In the murine Mycobacterium bovis Bacillus Calmette-Guérin (BCG) model we assessed whether early IL-12 production by macrophages depends on other cytokines. In vitro, murine bone marrow-derived macrophages produced IL-12 after infection with viable M. bovis BCG or stimulation with LPS, however, priming with recombinant interferon gamma (rIFN-gamma) was necessary. In addition, IL-12 production by these macrophages was blocked by specific anti-tumor necrosis factor alpha (TNF-alpha) antiserum. Macrophages from gene deletion mutant mice lacking either the IFN-gamma receptor or the TNF receptor 1 (p55) failed to produce IL-12 in vitro after stimulation with rIFN-gamma and mycobacterial infection. In vivo, IL-12 production was induced in spleens of immunocompetent mice early during M. bovis BCG infection but not in those of mutant mice lacking the receptors for IFN-gamma or TNF. Our results show that IL-12 production by macrophages in response to mycobacterial infection depends on IFN-gamma and TNF. Hence, IL-12 is not the first cytokine produced in mycobacterial infections.
Mots-clé
Animals, Base Sequence, Cells, Cultured, Female, Interferon-gamma/physiology, Interferon-gamma, Recombinant/pharmacology, Interleukin-12/biosynthesis, Macrophages/metabolism, Mice, Mice, Inbred C57BL, Molecular Sequence Data, Mycobacterium Infections/immunology, Mycobacterium bovis, Tumor Necrosis Factor-alpha/physiology
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/01/2008 12:36
Dernière modification de la notice
20/08/2019 14:11
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