[3H]PK 11195 and the localisation of secondary thalamic lesions following focal ischaemia in rat motor cortex.

Détails

ID Serval
serval:BIB_2C5B0E246006
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
[3H]PK 11195 and the localisation of secondary thalamic lesions following focal ischaemia in rat motor cortex.
Périodique
Neuroscience Letters
Auteur⸱e⸱s
Myers R., Manjil L.G., Frackowiak R.S., Cremer J.E.
ISSN
0304-3940 (Print)
ISSN-L
0304-3940
Statut éditorial
Publié
Date de publication
1991
Volume
133
Numéro
1
Pages
20-24
Langue
anglais
Notes
Publication types: Journal ArticlePublication Status: ppublish
Résumé
The peripheral-type benzodiazepine binding site (PTBBS) ligand, PK 11195, is known to be a marker of damage in the central nervous system, the binding being predominantly to macrophages. Using photochemically induced focal cortical ischaemia as a lesion model in the rat, we have investigated the detection of secondary lesions using [3H]PK 11195 and ex vivo autoradiography. Secondary lesions in the thalamus became apparent during the second week post-lesioning, at a time when [3H]PK 11195 binding around the primary lesion was beginning to subside. Using Brain Browser software, the identity of the labelled thalamic nucleus was confirmed, objectively, as the ventrolateral nucleus, known to have reciprocal connections with the lesioned cortical area. As with the primary lesion, high densities of PTBBS correlated with infiltration of macrophages. Three-dimensional reconstruction of [3H]PK 11195 autoradiograph images showed binding along white matter tracts between the primary and secondary lesions. We conclude that radiolabelled PK 11195 given in vivo can be used in the visualisation of secondary lesions and their associated degenerating tracts.
Mots-clé
Animals, Autoradiography, Brain Ischemia/pathology, Immunohistochemistry, Isoquinolines/diagnostic use, Male, Motor Cortex/pathology, Rats, Rats, Inbred Strains, Thalamic Nuclei/pathology, Thalamus/pathology, Tomography, Emission-Computed
Pubmed
Web of science
Création de la notice
25/09/2011 17:39
Dernière modification de la notice
20/08/2019 14:11
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