Radiolabeling of the hydrophobic components of lung surfactant with 3-(trifluoromethyl)-3-(m-[125I]iodophenyl)diazirine

Détails

ID Serval
serval:BIB_2C44AB587FEE
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Radiolabeling of the hydrophobic components of lung surfactant with 3-(trifluoromethyl)-3-(m-[125I]iodophenyl)diazirine
Périodique
Analytical Biochemistry
Auteur⸱e⸱s
Gilliard  N., Pappert  D., Merritt  T. A., Heldt  G., Spragg  R. G.
ISSN
0003-2697 (Print)
Statut éditorial
Publié
Date de publication
03/1991
Volume
193
Numéro
2
Pages
310-5
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Mar 2
Résumé
Studies of the metabolism and distribution of lung surfactant are aided by use of radiolabeled surfactant or surfactant components. These studies have often made use of [3H]- or [14C]phosphatidylcholine. Analysis of the lung content of surfactant containing these beta-emitting labels usually requires tissue digestion, use of scintillation fluids, and significant correction for quenching of photon production. Because use of a gamma-emitting isotope would obviate these requirements, we have investigated the use of 3-(trifluoromethyl)-3-(m-[125I]iodophenyl)diazirine ([125I]TID), a lipophilic photoactivatable compound, to radiolabel pulmonary surfactant. Our results indicate that, during photoactivation, products of [125I]TID are produced that result in radiolabeling of both the lipid and protein components of extracted porcine surfactant. Separation of radiolabeled surfactant from hydrophobic nonlabelling photolysis products was accomplished by gel chromatography. Exposure of surfactant (34 mumol/ml) to [125I]TID under labeling conditions resulted in incorporation of 45.3 +/- 5.1% of the radiolabel. Incorporation of radiolabel in the various phospholipids of lung surfactant was approximately equivalent. Lipophilic surfactant apoproteins were also radiolabeled. Finally, both in vitro and in vivo testing of radiolabeled surfactant (0.1 microCi/mg) revealed full retention of surface tension lowering ability.
Mots-clé
Animals Apoproteins/analysis Azirines/*diagnostic use Chromatography, Gel Chromatography, Thin Layer Electrophoresis, Polyacrylamide Gel Iodine Radioisotopes/*diagnostic use Isotope Labeling/methods Light Phospholipids/analysis *Pulmonary Surfactants/chemistry Swine
Pubmed
Web of science
Création de la notice
28/01/2008 10:50
Dernière modification de la notice
20/08/2019 13:11
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