Common and divergent roles for members of the mouse DCX superfamily

Détails

ID Serval
serval:BIB_2C0CDE66213A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Common and divergent roles for members of the mouse DCX superfamily
Périodique
Cell Cycle
Auteur(s)
Coquelle  F. M., Levy  T., Bergmann  S., Wolf  S. G., Bar-El  D., Sapir  T., Brody  Y., Orr  I., Barkai  N., Eichele  G., Reiner  O.
ISSN
1551-4005 (Electronic)
Statut éditorial
Publié
Date de publication
05/2006
Volume
5
Numéro
9
Pages
976-83
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May
Résumé
The doublecortin-like (DCX) domains serve as protein-interaction platforms. DCX tandem domains appear in the product of the X-linked doublecortin (DCX) gene, in retinitis pigmentosa-1 (RP1), as well as in other gene products. Mutations in the human DCX gene are associated with abnormal neuronal migration, epilepsy, and mental retardation; mutations in RP1 are associated with a form of inherited blindness, while DCDC2 has been associated with dyslectic reading disabilities. Motivated by the possible importance of this gene family, a thorough analysis to detect all family members in the mouse was conducted. The DCX-repeat gene superfamily is composed of eleven paralogs, and we cloned the DCX domains from nine different genes. Our study questioned which functions attributed to the DCX domain, are conserved among the different members. Our results suggest that the proteins with the DCX-domain have conserved and unique roles in microtubule regulation and signal transduction. All the tested proteins stimulated microtubule assembly in vitro. Proteins with tandem repeats stabilized the microtubule cytoskeleton in transfected cells, while those with single repeats localized to actin-rich subcellular structures, or the nucleus. All tested proteins interacted with components of the JNK/MAP-kinase pathway, while only a subset interacted with Neurabin 2, and a nonoverlapping group demonstrated actin association. The sub-specialization of some members due to confined intracellular localization, and protein interactions may explain the success of this superfamily.
Mots-clé
Amino Acid Sequence Animals Cloning, Molecular Conserved Sequence Mice Microtubule-Associated Proteins/chemistry/genetics/*physiology Microtubules/metabolism/ultrastructure Mitosis/physiology Multigene Family/*physiology Neuropeptides/chemistry/genetics/*physiology Protein Structure, Tertiary Sequence Alignment Signal Transduction/physiology
Pubmed
Web of science
Création de la notice
24/01/2008 14:10
Dernière modification de la notice
20/08/2019 13:11
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