Activity-dependent PSD formation and stabilization of newly formed spines in hippocampal slice cultures.

Détails

ID Serval
serval:BIB_2BF423B38F00
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Activity-dependent PSD formation and stabilization of newly formed spines in hippocampal slice cultures.
Périodique
Cerebral cortex
Auteur⸱e⸱s
De Roo M., Klauser P. (co-premier), Mendez P., Poglia L., Muller D.
ISSN
1460-2199 (Electronic)
ISSN-L
1047-3211
Statut éditorial
Publié
Date de publication
01/2008
Peer-reviewed
Oui
Volume
18
Numéro
1
Pages
151-161
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Development and remodeling of synaptic networks occurs through a continuous turnover of dendritic spines. However, the mechanisms that regulate the formation and stabilization of newly formed spines remain poorly understood. Here, we applied repetitive confocal imaging to hippocampal slice cultures to address these issues. We find that, although the turnover rate of protrusions progressively decreased during development, the process of stabilization of new spines remained comparable both in terms of time course and low level of efficacy. Irrespective of the developmental stage, most new protrusions were quickly eliminated, in particular filopodia, which only occasionally lead to the formation of stable dendritic spines. We also found that the stabilization of new protrusions was determined within a critical period of 24 h and that this coincided with an enlargement of the spine head and the expression of tagged PSD-95. Blockade of postsynaptic AMPA and NMDA receptors significantly reduced the capacity of new spines to express tagged PSD-95 and decreased their probability to be stabilized. These results suggest a model in which synaptic development is associated with an extensive, nonspecific growth of protrusions followed by stabilization of a few of them through a mechanism that involves activity-driven formation of a postsynaptic density.
Mots-clé
Animals, Dendritic Spines/physiology, Dendritic Spines/ultrastructure, Hippocampus/cytology, Hippocampus/physiology, Rats, Synapses/physiology, Synapses/ultrastructure, Tissue Culture Techniques
Pubmed
Web of science
Open Access
Oui
Création de la notice
07/02/2019 9:39
Dernière modification de la notice
03/12/2019 20:18
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