Human platelet lysate stimulated adipose stem cells exhibit strong neurotrophic potency for nerve tissue engineering applications.

Détails

ID Serval
serval:BIB_2BC121C5BBB7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Human platelet lysate stimulated adipose stem cells exhibit strong neurotrophic potency for nerve tissue engineering applications.
Périodique
Regenerative medicine
Auteur(s)
Lischer M., di Summa P.G., Oranges C.M., Schaefer D.J., Kalbermatten D.F., Guzman R., Madduri S.
ISSN
1746-076X (Electronic)
ISSN-L
1746-0751
Statut éditorial
Publié
Date de publication
03/2020
Peer-reviewed
Oui
Volume
15
Numéro
3
Pages
1399-1408
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Aim: We investigated a potential strategy involving human platelet lysate (HPL) as a media additive for enhancing the neurotrophic potency of human adipose stem cells (ASC). Materials & methods: Dorsal root ganglion explants, ASC and Schwann cells were used for in vitro axonal outgrowth experiments. Results: Remarkably, HPL-supplemented ASC promoted robust axonal outgrowth, in other words, four-times higher than fetal bovine serum-supplemented ASC and even matched to the level of Schwann cells. Further, analysis of regime of growth medium additive supplementation revealed the critical play of HPL in dorsal root ganglion and stem cells co-culture system for mounting effective axonal growth response. Conclusion: HPL supplementation significantly improved the neurotrophic potency of ASC as evidenced by the robust axonal outgrowth; these findings hold significance for nerve tissue engineering applications.
Mots-clé
Schwann cells, adipose stem cells, axonal regeneration, growth factors, human platelet lysate, nerve tissue engineering, neurotrophic factors
Pubmed
Web of science
Création de la notice
25/04/2020 18:32
Dernière modification de la notice
27/06/2020 5:20
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