Characterization of mesoangioblast cell fate and improved promyogenic potential of a satellite cell-like subpopulation upon transplantation in dystrophic murine muscles.

Détails

ID Serval
serval:BIB_2BB88877D39D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Characterization of mesoangioblast cell fate and improved promyogenic potential of a satellite cell-like subpopulation upon transplantation in dystrophic murine muscles.
Périodique
Stem cell research
Auteur(s)
Mavoungou L.O., Neuenschwander S., Pham U., Iyer P.S., Mermod N.
ISSN
1876-7753 (Electronic)
ISSN-L
1873-5061
Statut éditorial
Publié
Date de publication
12/2019
Peer-reviewed
Oui
Volume
41
Pages
101619
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Duchenne muscular dystrophy (DMD) is a lethal muscle-wasting disease caused by the lack of dystrophin in muscle fibers that is currently without curative treatment. Mesoangioblasts (MABs) are multipotent progenitor cells that can differentiate to a myogenic lineage and that can be used to express Dystrophin upon transplantation into muscles, in autologous gene therapy approaches. However, their fate in the muscle environment remains poorly characterized. Here, we investigated the differentiation fate of MABs following their transplantation in DMD murine muscles using a mass cytometry strategy. This allowed the identification and isolation of a fraction of MAB-derived cells presenting common properties with satellite muscle stem cells. This analysis also indicated that most cells did not undergo a myogenic differentiation path once in the muscle environment, limiting their capacity to restore dystrophin expression in transplanted muscles. We therefore assessed whether MAB treatment with cytokines and growth factors prior to engraftment may improve their myogenic fate. We identified a combination of such signals that ameliorates MABs capacity to undergo myogenic differentiation in vivo and to restore dystrophin expression upon engraftment in myopathic murine muscles.
Mots-clé
Animals, Cell Differentiation, Disease Models, Animal, Mice, Mice, Inbred mdx, Mice, SCID, Multipotent Stem Cells/metabolism, Multipotent Stem Cells/pathology, Muscular Dystrophy, Duchenne/genetics, Muscular Dystrophy, Duchenne/metabolism, Muscular Dystrophy, Duchenne/pathology, Muscular Dystrophy, Duchenne/therapy, Satellite Cells, Skeletal Muscle/metabolism, Satellite Cells, Skeletal Muscle/pathology, Satellite Cells, Skeletal Muscle/transplantation, Autologous transplantation, Mesoangioblast cells, Muscle dystrophies, Myogenic differentiation, Stem cell fate
Pubmed
Web of science
Open Access
Oui
Création de la notice
07/11/2019 23:49
Dernière modification de la notice
19/06/2020 6:21
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