Targeting Schwann cells by nonlytic arenaviral infection selectively inhibits myelination.

Détails

ID Serval
serval:BIB_2AF2C6FA0F20
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Targeting Schwann cells by nonlytic arenaviral infection selectively inhibits myelination.
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur(s)
Rambukkana A., Kunz S., Min J., Campbell K.P., Oldstone M.B.
ISSN
0027-8424 (Print)
ISSN-L
0027-8424
Statut éditorial
Publié
Date de publication
2003
Volume
100
Numéro
26
Pages
16071-16076
Langue
anglais
Résumé
Members of the arenavirus family, famous for their hemorrhagic syndromes, cause distinct neurological disorders; however, cellular and molecular targets as well as pathogenesis of peripheral nervous system disorders associated with these viruses are unknown. Using noncytolytic lymphocytic choriomeningitis virus, the prototype arenavirus, and pseudotyped Lassa fever virus, we showed that the Schwann cells, but not the neurons, were preferentially targeted and harbored the virus. This permissiveness was caused by the viral glycoprotein usage of its receptor alpha-dystroglycan, which was highly abundant on Schwann cell membranes. Persistent lymphocytic choriomeningitis virus infection rendered immature Schwann cells defective or incapable of forming compact myelin sheathes when they differentiated to myelinating phenotype in an in vitro differentiation model of Schwann cells. Persistent infection did not cause Schwann cell apoptosis or cytopathic effect. Defects in myelination coincided with the down-regulation of dystroglycan expression and disruption of the laminin-2 organization and basal lamina assembly on Schwann cell-axon units. The data provide evidence for a selective perturbation of laminin-2-laminin-2 receptor communication pathway in the peripheral nervous system by a nonlytic virus and the resulting myelin defects, which may partly contribute to neurological abnormalities associated with arenaviral infection.
Mots-clé
Animals, Arenaviridae Infections/physiopathology, Binding, Competitive, Cells, Cultured, Coculture Techniques, Cytoskeletal Proteins/metabolism, Dystroglycans, Ganglia, Spinal/physiology, Ganglia, Spinal/virology, Humans, Laminin/metabolism, Lymphocytic choriomeningitis virus/physiology, Membrane Glycoproteins/metabolism, Moloney murine leukemia virus/physiology, Myelin Sheath/physiology, Rats, Schwann Cells/physiology, Schwann Cells/virology
Pubmed
Web of science
Open Access
Oui
Création de la notice
17/04/2013 11:56
Dernière modification de la notice
20/08/2019 13:10
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