Oxygen uptake during early cardiogenesis of the chick.

Détails

ID Serval
serval:BIB_2AEA706391AA
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Oxygen uptake during early cardiogenesis of the chick.
Périodique
American Journal of Physiology. Heart and Circulatory Physiology
Auteur(s)
Raddatz E., Servin M., Kucera P.
ISSN
0363-6135 (Print)
ISSN-L
0002-9513
Statut éditorial
Publié
Date de publication
1992
Volume
262
Numéro
4 Pt 2
Pages
H1224-H1230
Langue
anglais
Résumé
Oxidative metabolism of the isolated embryonic heart of the chick has been determined using a spectrophotometric technique allowing global as well as localized micromeasurements of the O2 uptake. Entire hearts, excised from embryos of 10 somites (primordia fused, stage 10 HH) and 40 somites (S shaped, stage 20 HH) were placed in a special chamber under controlled metabolic conditions where they continued to beat spontaneously and regularly. During the 32 h of development, the O2 consumption of the whole heart increased from 0.9 +/- 0.1 to 5.3 +/- 0.8 nmol O2/h. These values corrected for protein content were, however, comparable (0.45 nmol O2.h-1.micrograms-1). At stage 10-12, the O2 uptake varied along the cardiac tube (from 0.74 to 1.0 nmol O2.h-1.mm-2). From stage 10 to 20, the O2 uptake per unit area of ventricle wall increased from 0.7 +/- 0.2 to 1.8 +/- 0.2 nmol O2.h-1.mm-2, and the O2 uptake per myocardial volume during one cardiac cycle varied from 7 to 2.5 nmol O2/cm3. These results indicate that, despite an intense morphogenesis, the cardiac tissue has a rather low and stable oxidative metabolism, although the O2 requirement of the whole heart increases significantly. Moreover, the normalized suprabasal aerobic energy expenditure decreases throughout early cardiogenesis. The functional integrity of the isolated embryonic heart combined with the experimental possibilities of the microtechnique make the preparation appropriate for studying the changes in cardiac metabolism during development.
Mots-clé
Animals, Chick Embryo, Embryonic and Fetal Development, Fetal Heart/anatomy & histology, Fetal Heart/metabolism, Heart Rate, Heart Ventricles, Oxidation-Reduction, Oxygen Consumption, Spectrophotometry/methods
Pubmed
Web of science
Création de la notice
24/01/2008 13:19
Dernière modification de la notice
20/08/2019 13:10
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