Differential requirement for CD4 help in the development of an antigen-specific CD8+ T cell response depending on the route of immunization.

Détails

ID Serval
serval:BIB_2AE8D3D2C0D6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Differential requirement for CD4 help in the development of an antigen-specific CD8+ T cell response depending on the route of immunization.
Périodique
Journal of immunology
Auteur(s)
Bour H., Horvath C., Lurquin C., Cerottini J.C., MacDonald H.R.
ISSN
0022-1767
Statut éditorial
Publié
Date de publication
1998
Peer-reviewed
Oui
Volume
160
Numéro
11
Pages
5522-5529
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Previous studies in our laboratory have shown that DBA/2 mice injected i.p. with syngeneic P815 tumor cells transfected with the HLA-CW3 gene (P815-CW3) showed a dramatic expansion of activated CD8+CD62L- T cells expressing exclusively the Vbeta10 segment. We have used this model to study the regulatory mechanisms involved in the development of the CW3-specific CD8+ response, with respect to different routes of immunization. Whereas both intradermal (i.d.) and i.p. immunization of DBA/2 mice with P815-CW3 cells led to a strong expansion of CD8+CD62L-Vbeta10+ cells, only the i.d. route allowed this expansion after immunization with P815 cells transfected with a minigene coding for the antigenic epitope CW3 170-179 (P815 miniCW3). Furthermore, depletion of CD4+ T cells in vivo completely abolished the specific response of CD8+CD62L-Vbeta10+ cells and prevented the rejection of P815-CW3 tumor cells injected i.p., whereas it did not affect CD8S+CD62L-Vbeta10+ cell expansion after i.d. immunization with either P815-CW3 or P815 miniCW3. Finally, the CW3-specific CD8+ memory response was identical whether or not CD4+ T cells were depleted during the primary response. Collectively, these results suggest that the CD8+ T cell response to P815-CW3 tumor cells injected i.p. is strictly dependent upon recognition of a helper epitope by CD4+ T cells, whereas no such requirement is observed for i.d. injection.
Mots-clé
Adoptive Transfer/methods, Animals, CD8-Positive T-Lymphocytes/immunology, CD8-Positive T-Lymphocytes/metabolism, Cytotoxicity, Immunologic, Epitopes, T-Lymphocyte/immunology, Female, Genes, MHC Class I, HLA-C Antigens/administration &amp, dosage, HLA-C Antigens/immunology, Immunologic Memory, Injections, Intradermal, Injections, Intraperitoneal, Kinetics, L-Selectin/analysis, Lymphocyte Activation, Mast-Cell Sarcoma, Mice, Mice, Inbred DBA, Mice, Nude, Neoplasm Transplantation, Receptors, Antigen, T-Cell, alpha-beta/analysis, T-Lymphocyte Subsets/immunology, T-Lymphocyte Subsets/metabolism, T-Lymphocytes, Cytotoxic/immunology, T-Lymphocytes, Cytotoxic/metabolism, T-Lymphocytes, Helper-Inducer/immunology, Transfection/immunology, Tumor Cells, Cultured
Pubmed
Web of science
Création de la notice
28/01/2008 11:14
Dernière modification de la notice
20/08/2019 13:10
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