Engineered acellular collagen scaffold for endogenous cell guidance, a novel approach in urethral regeneration.

Détails

ID Serval
serval:BIB_2ACC4DEA810F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Engineered acellular collagen scaffold for endogenous cell guidance, a novel approach in urethral regeneration.
Périodique
Acta biomaterialia
Auteur(s)
Pinnagoda K., Larsson H.M., Vythilingam G., Vardar E., Engelhardt E.M., Thambidorai R.C., Hubbell J.A., Frey P.
ISSN
1878-7568 (Electronic)
ISSN-L
1742-7061
Statut éditorial
Publié
Date de publication
01/10/2016
Peer-reviewed
Oui
Volume
43
Pages
208-217
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
The treatment of congenital malformations or injuries of the urethra using existing autologous tissues can be associated with post-operative complications. Using rat-tail collagen, we have engineered an acellular high-density collagen tube. These tubes were made of 2 layers and they could sustain greater burst pressures than the monolayered tubes. Although it remains a weak material this 2 layered tube could be sutured to the native urethra. In 20 male New Zealand white rabbits, 2cm long grafts were sutured in place after subtotal excision of the urethra. This long-term study was performed in Lausanne (Switzerland) and in Kuala Lumpur (Malaysia). No catheter was placed post-operatively. All rabbits survived the surgical implantation. The animals were evaluated at 1, 3, 6, and 9months by contrast voiding cysto-urethrography, histological examination and immunohistochemistry. Spontaneous re-population of urothelial and smooth muscle cells on all grafts was demonstrated. Cellular organization increased with time, however, 20% of both fistula and stenosis could be observed post-operatively. This off-the shelf scaffold with a promising urethral regeneration has a potential for clinical application.
In this study we have tissue engineered a novel cell free tubular collagen based scaffold and used it as a urethral graft in a rabbit model. The novelty of our technique is that the tube can be sutured. Testing showed better burst pressures and the grafts could then be successfully implanted after a urethral excision. This long term study demonstrated excellent biocompatibility of the 2cm graft and gradual regeneration with time, challenging the current literature. Finally, the main impact is that we describe an off-the-shelf and cost-effective product with comparable surgical outcome to the cellular grafts.

Mots-clé
Animals, Collagen/pharmacology, Immunohistochemistry, Implants, Experimental, Male, Materials Testing, Rabbits, Rats, Regeneration/drug effects, Tissue Engineering/methods, Urethra/drug effects, Urethra/pathology, Urethra/physiology, Urethra/surgery, Acellular, Collagen, Rabbit, Tissue engineered, Tubular, Urethral graft
Pubmed
Web of science
Création de la notice
30/07/2016 10:47
Dernière modification de la notice
20/08/2019 13:10
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